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Dietary recommendations during the COVID-19 pandemic. Statement of the Committee of Human Nutrition Science of the Polish Academy of Sciences
Our new CiteScore2020 from SCOPUS
June 2021 Issue has just been published
September 2021 issue has just been released
Volume 71's Reviewers Index
Our new Impact Factor and Cite Score
Our new CiteScore2022 from SCOPUS
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NEW RAPID METHOD OF CACO-2 CELL DIFFERENTIATION
Publication date: 2003-03-31
Pol. J. Food Nutr. Sci. 2003;53(Special issue 1s):60-64
KEYWORDS
ABSTRACT
The Caco-2 cell line is used by many investigators as a cellular intestinal model. The aim of this work was to improve the conventional long-term 21-day Caco-2 cell culture to obtain a simple, less expensive and more rapid culture system. In the new culture system, the cells were grown
on polycarbonate filters in serum-reduced medium containing 2% iron-saturated calf serum supplemented with combination of growth factors and hormones. The modified culture conditions were designed to induce rapid differentiation of Caco-2 cells. Using this system, the differentiated enterocyte-like monolayer was established within 4-6 days and characterized for cell morphology, growth rate, glucose consumption rate, lactate production rate, the monolayer integrity and activity of alkaline phosphatase.
| eISSN: | 2083-6007 |
| ISSN: | 1230-0322 |
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