Furosemide therapy lowers pathologically raised blood pressure. However, furosemide causes hypercalciuria. This study was designed to determine the diuretic effect of furosemide on bone minerals (Ca, Mg and K) in right tibial bone of male rats and the possible positive impact of vitamin D, oligofructose (FOS), and lactose against furosemide’s diuretic effect on these minerals. The experiment was conducted on 30 male albino rats that were divided into 5 groups of similar mean body weights. Groups 1 and 2 were fed on basal diet. Group 3 was fed on basal diet supplemented with 800 mg of vitamin D. Groups 4 and 5 were fed on basal diet containing 10% FOS and 10% lactose, respectively. Groups 2, 3, 4 and 5 were treated with an oral administration of furosemide (15 mg/kg/day). Rats had diet and drinking water provided ad libitum for 4 weeks. The results showed that furosemide caused significantly (p<0.05) increased urinary excretion of Ca (245%), Mg (155%) and K (363%) compared to the control. Supplementation of vitamin D, FOS and lactose led to a decrease in Ca, Mg and K excretion in urine compared to the furosemide group. Mean Ca level in plasma and tibia was significantly (p<0.05) lower in the furosemide group compared to the control one. Supplementation of vitamin D and FOS raised Ca content in tibia to normal levels. We concluded that vitamin D and FOS supplementation has a protective action against hypercalciuria and bone loss of furosemide diuretic therapy in rats.