We tested the effect of aluminum sulphate on serum protein profile, liver enzyme activities, bilirubin levels, total protein content and the efficiency of vitamin C to reduce the adverse effect of aluminum sulphate. Mice were treated orally for 1, 2 and 3 weeks with three doses of aluminum sulphate 387.5, 775 and 1550 mg/kg b.wt. representing 1/16, 1/8 and 1/4 LD₅₀, respectively. Significant reduction in serum total protein content and protein fraction concentrations is noticed except serum albumin which recorded a significant increase in a dose-dependent relationship of aluminum sulphate through follow up of time. Liver function enzyme activities and bilirubin levels recorded significantly elevated levels with 1/4, 1/8 and 1/16 LD₅₀ of aluminum sulphate at 1^st, 2^nd and 3^rd weeks of treatment. Concurrent administration of vitamin C at a dose of 20 mg/kg b.wt. to healthy mice produced a significant increase in serum total protein content and protein fraction concentrations. No effect of vitamin C on bilirubin levels and liver function enzyme activities in healthy animals was observed. On the other hand, vitamin C ameliorated serum total protein content and protein fraction concentrations, bilirubin levels and liver function enzyme activities in mice when administrated with 1/4 LD₅₀ of aluminum sulphate. Concerning the effect of different doses of aluminum sulphate within weeks, ANOVA test revealed significant variation among each group except normal control group which recorded insignificant change after 1^st, 2^nd, and 3^rd week. In vitamin C-administrated healthy mice, only albumin concentration and bilirubin levels recorded a non significant variations within different weeks.