RAPID CHROMATOGRAPHIC METHOD FOR SEPARATION OF GREEN TEA PROANTHOCYANIDINS

A rapid chromatographic method for the separation of proanthocyanidins from the phenolic extract of green tea was described. A crude catechin mixture from green tea was applied onto a Toyopearl HW-40S column (TosoHaas). Six fractions (I-VI) were eluted from the column using ethanol as a mobile phase. Fractions I-III were free of catechins. Catechins (EC, ECG, EGC, EGCG) were present in fractions IV-VI. Two proanthocyanidin fractions (VII-VIII) were eluted from the column with acetone-water (1:1; v/v). Proanthocyanidins present in these fractions exhibited a strong positive vanillin reaction. The absence of catechins in the proanthocyanidin fractions was confirmed by means of thin layer chromatography.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.