SYNTHESIS, PROPERTIES, AND APPLICATION OF LIPASE FROM  CANDIDA ANTARCTICA  FOR HIGH YIELD MONOACYLGLYCEROL BIOSYNTHESIS.

Synthesis of extracellular lipases by Candida antarctica was induced by adding fat or oil to the culture medium. The highest lipase activity (9.1 U) was obtained after 72 h of cultivation of yeast in medium supplemented with 2% (v/v) 1,2,3-trioleoyl-sn-glycerol. On the basis of SDS-PAGE analysis, Candida antarctica synthesized two forms of lipase having molecular weight of 36 and 52 kDa. Lipase from Candida antarctica showed the highest activity at pH 8.0 and temperature of 35^oC and was stable in this range of temperature and pH. Activity of Candida antarctica lipase was higher on substrates having short-chain and medium-chain fatty acids than on these containing long-chain fatty acids. Using Candida antarctica lipase immobilized on Celite 545 in the synthesis of monoacylglycerols, 100% of conversion was obtained after esterification of oleic acid with isopropylidene glycerol. After hydrolysis of protected monoacylglycerols with trifluoroacetic acid, pure monoacylglycerols were obtained.

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SELECTED PHYSICOCHEMICAL PROPERTIES OF FATTY ACID ESTERS WITH MONO- AND DISACCHARIDES

ACTIVITY AND IMMUNOREACTIVITY OF LIPASE IN STORED SUNFLOWER SEEDS

EFFECT OF NATIVE ENZYMES ON THE QUALITY OF LIPIDS IN EXPERIMENTALLY-STORED RAPESEED

PROPERTIES OF IMMOBILIZED AND FREE LIPASE FROM RHIZOPUS COHNII

SOME PROPERTIES OF LIPASE PREPARATION FROM RHIZOPUS COHNII

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.