Antioxidant Vitamins as Oxidative Stress Markers in Rat Plasma After Physical Exercise - a Short Report
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Publication date: 2014-12-31
Pol. J. Food Nutr. Sci. 2014;64(4):277–281
The aim of the study was to verify the hypothesis that β-carotene, vitamin E, vitamin C administered individually or in combination may differently modify their levels in blood plasma being also markers of the oxidative stress. Male Wistar rats were supplemented antioxidants per os (α-tocopherol - 2 mg/d, ascorbic acid – 12 mg/d, β-carotene – 1 mg/d), both individually or in combination of 2 or 3, for 14 days. During experiment half of the animals in each group (n=8) were subjected by treadmill exercise for 15 minutes at the speed 20 m/min, to cause oxidative stress. Vitamins in rat plasma were assessed by the high-performance liquid chromatography (HPLC). Results suggest that vitamin E and C supplemented simultaneously may provide some benefit during physical exercise. The significant influence of administered α-tocopherol acetate and physical exercise on the level of α-tocopherol in the plasma was observed. Thus only the concentration of α-tocopherol in blood may be treated as a marker of oxidative stress.
α-Tocopherol Protects the Heart, Muscles, and Testes from Lipid Peroxidation in Growing Male Rats Subjected to Physical Efforts
Magdalena Górnicka, Anna Ciecierska, Jadwiga Hamulka, Małgorzata Drywień, Joanna Frackiewicz, Krzysztof Górnicki, Agata Wawrzyniak
Oxidative Medicine and Cellular Longevity