BIOSYNTHESIS OF AMYLASES BY BACILLUS POLYMYXA CELLS IMMOBILIZED IN CALCIUM ALGINATE AND CHITOSANE.
 
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Pol. J. Food Nutr. Sci. 1999;49(1):45–52
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ABSTRACT
The aim of the study was to determine optimal conditions for amylases biosynthesis by immobilized Bacillus polymyxa B-20 cells. Composition of the medium and immobilization conditions to a large extent affected the efficiency of enzyme production. Optimal production medium consisted of yeast extract (5-10 g/L), maltose, peptone and mineral salts. Stability of alginate biocatalyst beads was increased by 30 g/L gel concentration, increased bead size and chemical gel modification by glutaraldehyde. Increased quantities of synthesized enzymes were proportional to the increment of initial biomass present in beads, ant to a lesser degree, depended on quantities of immobilized inoculum. In repeated batch culture, the bacteria immobilized in alginate were able to synthesize 32% more enzymes than those immobilized in chitosane. Operational stability of alginate and chitosane beads was maintained for 17 days.
ISSN:1230-0322