CONTENT OF PROANTHOCYANIDINS IN SELECTED PLANT EXTRACTS AS DETERMINED VIA N-BUTANOL/HCL HYDROLYSIS AND A COLORIMETRIC ASSAY OR BY HPLC – A SHORT REPORT

Ryszard Amarowicz; Ronald B. Pegg

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CONTENT OF PROANTHOCYANIDINS IN SELECTED PLANT EXTRACTS AS DETERMINED VIA N-BUTANOL/HCL HYDROLYSIS AND A COLORIMETRIC ASSAY OR BY HPLC – A SHORT REPORT

Ryszard Amarowicz

Ronald B. Pegg

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Publication date: 2006-09-30

Pol. J. Food Nutr. Sci. 2006;56(3):319-322

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Extracts of phenolic compounds were prepared from peas, faba beans, broad beans, red beans, adzuki beans, vetch, red lentils, green lentils, hazelnuts, walnuts and almonds using 80% (v/v) acetone. The content of proanthocyanidins was determined after n-butanol/HCl hydrolysis using a colorimetric assay and by an HPLC method. Extracts of red beans and adzuki beans were characterised by the highest content of proanthocyanidins. The hydrolysis of extracts of legume seeds, hazelnuts, walnuts and almonds resulted in delphinidin and cyanidin formation. A strong correlation (r = 0.9877) was found between the content of proanthocyanidins in plant extracts determined using a colorimetric assay and by an HPLC method.

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W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.