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Dietary Chicory Inulin-Rich Meal Exerts Greater Healing Effects than Fructooligosaccharide Preparation in Rats with Trinitrobenzenesulfonic Acid-Induced Necrotic Colitis
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BIOLOGICAL FUNCTION OF FOOD, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Poland
IMMUNOLOGY AND FOOD MICROBIOLOGY, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Poland
PHARMACOLOGY AND TOXICOLOGY, University of Warmia and Mazury, Faculty of Medicine, Poland
BIOCHEMISTRY AND TOXICOLOGY, Animal Sciences and Bioeconomy, University of Life Sciences, Faculty of Biology, Poland
Submission date: 2018-11-16
Final revision date: 2019-02-08
Acceptance date: 2019-02-21
Online publication date: 2019-03-11
Publication date: 2019-03-26
Corresponding author
Joanna Fotschki   

IMMUNOLOGY AND FOOD MICROBIOLOGY, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10 Str, 10-748, Olsztyn, Poland
Pol. J. Food Nutr. Sci. 2019;69(2):147-155
The aim of the study was to compare the effects of chicory root inulin-rich meal (containing polyphenolic fraction as well) and pure fructooligosaccharides (FOS) on necrotic colitis induced with trinitrobenzenesulfonic acid (TNBS) in Wistar rats. Both chicory preparations significantly reduced the pH of colonic digesta and favourably lowered the caecal activity of bacterial β-glucuronidase as well as the caecal concentration of putrefactive short-chain fatty acids in comparison to the control TNBS rats. Both chicory preparations enhanced the production of total short-chain fatty acid (SCFA pool) and concentration of anti-inflammatory propionic acid in the caecal digesta. Nevertheless, only dietary chicory meal favourably increased the total SCFA concentration and thus decreased the pH of caecal digesta. Increased caecal SCFA production may explain observed greater reduction in mucosal necrosis and increase in glandular mucosal regeneration in rats fed a diet with chicory root meal. Both chicory preparations beneficially regulated physiological parameters in the lower part of the rat intestinal tract after TNBS-induced colitis, however the dietary treatment with chicory meal showed stronger reduction of mucosal disturbances caused by colitis. Those beneficial effects might be related to higher polymerization of inulin vs. FOS and presence of biologically active compounds in the meal, i.e., phenolic compounds, which have strong impact on intestinal microbial activity and thus indirectly alleviated mucosal disturbances caused by colitis.
Barcelo, A., Claustre, J., Moro, F., Chayvialle, J., Cuber, J., Plaisancie, P. (2000). Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon. Gut, 46(2), 218–224.
Cardona, M.E., Collinder, E., Stern, S., Tjellström, B., Norin, E., Midtvedt, T. (2005). Correlation between fecal iso-butyric and iso-valeric acids in different species. Microbial Ecology in Health and Disease, 17(3), 177–182.
Cherbut, C., Michel, C., Lecannu, G. (2003). The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats. The Journal of Nutrition, 133(1), 21–27.
De Pascual-Teresa, S., Santos-Buelga, C., Rivas-Gonzalo, J.C. (2000). Quantitative analysis of flavan-3-ols in Spanish food stuffs and beverages. Journal of Agricultural and Food Chemistry, 49(11), 5331-5337.
Debnath, T., Kim, D.H., Lim, B.O. (2013). Natural products as a source of anti-inflammatory agents associated with inflammatory bowel disease. Molecules, 18(6), 7253–7270.
Dell, R.D., Holleran, S., Ramakrishnan, R. (2002). Sample size determination. ILAR Journal, 43(4), 207–213.
Engel, M.A., Kellermann, C.A., Rau, T., Burnat, G., Hahn, E.G., Konturek, P.C. (2008). Ulcerative colitis in AKR mice is attenuated by intraperitoneally administered anandamide. Journal of Physiology Pharmacology, 59(4), 673–689.
Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes (2010). Official Journal of the European Union, L 276, 20.10.2010, p. 33–79.
Fischer, A.H., Jacobson, K.A., Rose, J., Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harbor Protocols, 1;2008:pdb.prot4986. [].
Fotschki, B., Juśkiewicz, J., Sojka, M., Jurgoński, A., Zduńczyk, Z. (2015). Ellagitannins and flavan-3-ols from raspberry pomace modulate caecal fermentation processes and plasma lipid parameters in rats. Molecules, 20(12), 22848–22962.
Fotschki, B., Milala, J., Jurgoński, A., Karlińska, E., Zduńczyk, Z., Juśkiewicz, J. (2014). Strawberry ellagitannins thwarted the positive effects of dietary fructooligosaccharides in rat cecum. Journal of Agricultural and Food Chemistry, 62(25), SI, 5871–5880.
Ganaie, M.A., Lateef, A., Gupta, U.S. (2014). Enzymatic trends of fructooligosaccharides production by microorganisms. Applied Biochemistry and Biotechnology,172(4), 2143–2159.
Gibson, G.R., Beatty, E.R., Wang, X., Cummings, J.H. (1995). Selective stimulation of the bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology, 108(4), 975–982.
Gonçalves, C.C.M., Hernandes, L., Bersani-Amado, C.A., Franco, S.L., Silva, J.F.D.S., Natali, M.R.M. (2013). Use of propolis hydroalcoholic extract to treat colitis experimentally induced in rats by 2,4,6-trinitrobenzenesulfonic acid. Evidence-Based Complementary and Alternative Medicine, 2013, art. no. 853976.
Hamer, H.M., Jonkers, D., Venema, K., Vanhoutvin, S., Troost, F.J., Brummer, R.J. (2008). Review article: The role of butyrate on colonic function. Alimentary Pharmacology & Therapeutics, 27(2), 104–119.
Hofirek, B., Haas, D. (2001). Comparative studies of ruminal fluid collected by oral tube or by puncture of the caudorental ruminal sac. Acta Veterinaria Brno, 70(1), 27-33.
Jurgoński, A., Fotschki, B., Juśkiewicz, J. (2015). Disparate metabolic effects of blackcurrant seed oil in rats fed a basal and obesogenic diet. European Journal of Nutrition, 54(6), 991–999.
Jurgoński, A., Milala, J., Juśkiewicz, J., Zduńczyk, Z., Król, B. (2011). Composition of chicory root, peel, seed and leaf ethanol extracts and biological properties of their non-inulin fractions. Food Technology and Biotechnology, 49(1), 40–47.
Juśkiewicz, J., Milala, J., Jurgoński, A., Król, B., Zduńczyk, Z. (2011a). Consumption of polyphenol concentrate with dietary fructo-oligosaccharides enhance cecal metabolizm of quercetin glycosides in rats. Nutrition, 27(3), 351–357.
Juśkiewicz, J., Zduńczyk, Z., Żary-Sikorska, E., Król, B., Milala, J., Jurgoński, A. (2011b). Effect of the dietary polyphenolic fraction of chicory root, peel, seed and leaf extracts on caecal fermentation and blood parameters in rats fed diets containing prebiotic fructans. The British Journal of Nutrition, 105(5), 710–720.
Klewicka, E., Zduńczyk, Z., Juśkiewicz, J. (2009). Effect of lactobacillus fermented beetroot juice on composition and activity of cecal microflora in rats. European Food Research and Technology, 229(1), 153–157.
Kosmala, M., Jurgoński, A., Juśkiewicz, J., Karlińska, E., Macierzyński, J., Rój, E., Zduńczyk, Z. (2017). Chemical composition of blackberry press cake, polyphenolic extract, and defatted seeds, and their effects on cecal fermentation, bacterial metabolites, and blood lipid profile in rats. Journal of Agricultural and Food Chemistry, 65(27), 5470–5479.
Kosmala, M., Zduńczyk, Z., Juśkiewicz, J., Jurgoński, A., Karlińska, E., Macierzyński, J., Jańczak, R., Rój, E. (2015). Chemical composition of defatted strawberry and raspberry seeds and the effect of these dietary ingredients on polyphenol metabolites, intestinal function, and selected serum parameters in rats. Journal of Agricultural and Food Chemistry, 63(11), 2989–2996.
Kosmala, M., Zduńczyk, Z., Kołodziejczyk, K., Klimczak, E., Juśkiewicz, J., Zduńczyk, P. (2014). Chemical composition of polyphenols extracted from strawberry pomace and their effect on physiological properties of diets supplemented with different types of dietary fibre in rats. European Journal of Nutrition, 53(2), 521–532.
Lahouar, L., Pochart, P., Salem, H.B., El Felah, M., Mokni, M., Magne, F., Mangin, I., Suau, A., Pereira, E., Hammami, M., Achour, L. (2012). Effect of dietary fibre of barley variety “Rihane” on azoxymethane-induced aberrant crypt foci development and on colonic microbiota diversity in rats. The British Journal of Nutrition, 108(11), 2034–2042.
Lara-Villoslada, F., Debras, E., Nieto, A., Concha, A., Gálvez, J., López-Huertas, E., Boza, J., Obled, C., Xaus, J. (2006). Oligosaccharides isolated from goat milk reduce intestinal inflammation in a rat model of dextran sodium sulfate-induced colitis. Clinical Nutrition, 25(3), 477–488.
Liu, X., Wang, J. (2011). Anti-inflammatory effects of iridoid glycosides fraction of Folium syringae leaves on TNBS-induced colitis in rats. Journal of Ethnopharmacology, 133(2), 780–787.
Louis, P., Flint, H.J. (2017). Formation of propionate and butyrate by the human colonic microbiota. Environmental Microbiology, 19(1), 29–41.
Morris, G.P., Beck, P.L., Herridge, M.S., Depew, W.T., Szewczuk, M.R., Wallace, J.L. (1989). Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology, 96(3),795–803.
Motavallian-Naeini, A., Andalib, S., Rabbani, M., Mahzouni, P., Afsharipour, M., Minaiyan, M. (2012). Validation and optimization of experimental colitis induction in rats using 2, 4, 6-trinitrobenzene sulfonic acid. Research in Pharmaceutical Sciences, 7(3), 159–169.
Ramirez-Farias, C., Slezak, K., Fuller, Z., Duncan, A., Holtrop, G., Louis, P. (2009). Effect of inulin on the human gut microbiota: stimulation of Bifidobacterium adolescentis and Faecalibacterium prausnitzii. The British Journal of Nutrition, 101(4), 541–550.
Roberton, A.M., Stanley, R.A., Lee, S.P., Lindop, R., Thomsen, L., Jones, C.T. (1982). Biliary control of β-glucuronidase activity in the luminal contents of the rat ileum, cecum, and rectum. Cancer Research, 42(12), 5165–5166.
Rodríguez-Cabezas, M.E., Gálvez, J., Lorente, M.D., Concha, A., Camuesco, D., Azzouz, S., Osuna, A., Redondo, L., Zarzuelo, A. (2002). Dietary Fiber Down-Regulates Colonic Tumor Necrosis Factor α and Nitric Oxide Production in Trinitrobenzenesulfonic Acid-Induced Colitic Rats. The Journal of Nutrition, 132(11), 3263–3271.
Shimotoyodome, A., Meguro, S., Hase, T., Tokimitsu, I., Sakata, T. (2000). Short chain fatty acids but not lactate or succinate stimulate mucus release in the rat colon. Comparative biochemistry and physiology. Part A, Molecular & Integrative Physiology, 125(4), 525–531.
Singh, R.S., Singh, R.P., Kennedy, J.F. (2016). Recent insights in enzymatic synthesis of fructooligosaccharides from inulin. International Journal of Biological Macromolecules, 85, 565–572.
Sturm, A., Dignass, A.U. (2008). Epithelial restitution and wound healing in inflammatory bowel disease. World Journal of Gastroenterology, 14(3), 348–353.
Topping, D.L., Clifton, P.M. (2001). Short-chain fatty acids and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiological Reviews, 81(3), 1031–1064.
van der Beek, C.M., Dejong, C.H.C., Troost, F.J., Masclee, A.A.M., Lenaerts, K. (2017). Role of short-chain fatty acids in colonic inflammation, carcinogenesis, and mucosal protection and healing. Nutrition Reviews, 75(4), 286–305.
Vinolo, M.A.R., Rodrigues, H.G., Nachbar, R.T., Curi, R. (2011). Regulation of inflammation by short chain fatty acids. Nutrients, 3(10), 858–876.
Wang, L., Liu, H.M., Xie, A.J., Zhu, C.Y., Qin, G.Y. (2018). Dietary Fiber Extraction from Defatted Corn Hull by Hot-Compressed Water. Polish Journal of Food and Nutrition Sciences, 68(2), 133–140.
Witaicenis, A., Fruet, A.C., Salem, L., Di Stasi, L.C. (2010). Dietary polydextrose prevents inflammatory bowel disease in trinitrobenzenesulfonic acid model of rat colitis. Journal of Medicinal Food, 13(6), 1391–1396.
Yajima, T., Inoue, R., Matsumoto, M., Yajima, M. (2011). Non-neuronal release of ACh plays a key role in secretory response to luminal propionate in rat colon. The Journal of Physiology, 589(4), 953–962.
Yue-Meng, W., You-Qing, Z., Bing, X., Jun, L. (2011). Treating TNBS-induced colitis in rats with probiotics. Turkish Journal of Gastroenterology, 22(5), 486–493.
Żary-Sikorska, E., Juśkiewicz, J. (2008). Effect of fructans with different degrees of polymerization on bacterial enzymes activity, lipid profile and antioxidant status in rats. Polish Journal of Food and Nutrition Sciences, 58(2), 269–272.
Żary-Sikorska, E., Juśkiewicz, J., Jundziłł, A., Rybka, J. (2016). Effects of diets varying in the type of dietary fibre and its combination with polyphenols on gut function, microbial activity and antioxidant status in rats. Journal of Animal and Feed Sciences, 25(3), 250–258.
Zhang, Y-Z., Li Y-Y. (2014). Inflammatory bowel disease: pathogenesis. World Journal of Gastroenterology, 20(1), 91–99.
Zhong, J., Luo, B.Y., Xiang, M.J., Liu, H.W., Zhai, Z.K., Wang, T.S., Craig, S.A.S. (2000). Studies on the effects of polydextrose intake on physiologic functions in Chinese people. The American Journal of Clinical Nutrition, 72(6), 1503–1509.
Zorrilla, P., Rodriguez-Nogales, A., Algieri, F., Garrido-Mesa, N., Olivares, M., Rondón, D., Zarzuelo, A., Utrilla, M.P., Galvez, J., Rodriguez-Cabezas, M.E. (2014). Intestinal anti-inflammatory activity of the polyphenolic-enriched extract Amanda® in the trinitrobenzenesulphonic acid model of rat colitis. Journal of Functional Foods, 11, 449–459.
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