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Effects of Long-Term Dietary Administration of Kale (Brassica oleracea L. var. acephala DC) Leaves on the Antioxidant Status and Blood Biochemical Markers in Rats
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Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, 4 Święcickiego Str., 60-781 Poznań, Poland
Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd Str., 60-631 Poznań, Poland
Department of Food Technology of Plant Origin, Poznan University of Life Sciences, 31 Wojska Polskiego Str., 60-624 Poznan, Poland
Submission date: 2022-05-19
Final revision date: 2022-07-17
Acceptance date: 2022-07-27
Online publication date: 2022-08-11
Publication date: 2022-09-05
Corresponding author
Katarzyna Papierska   

Department of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcickiego 4 St., 60-781, Poznan, Poland
Pol. J. Food Nutr. Sci. 2022;72(3):239-247
Kale (Brassica oleracea L. var. acephala DC) is a leafy green vegetable which belongs to the Brassicaceae family, one of the most commonly cultivated and consumed edible plants. The aim of this study was to investigate the potential adverse effects and antioxidant properties of freeze-dried kale leaves in 90-day dietary experiment on Wistar rats. The kale leaf powder was added to a diet at levels: 10, 30, and 60 g/kg feed. The standard blood biochemical and hematological markers, antioxidant enzyme activities, level of lipid peroxidation, reduced glutathione content, and damage to DNA in the liver were measured. Antioxidant potential in serum was measured and expressed as Trolox equivalents antioxidant capacity (TEAC). The kale leaf phytochemicals, i.e., glucosinolates, phenolic acids and flavonols, were quantified. Major glucosinolates were glucoiberin (164.6 mg/100 g d.w.) and glucobrassicin (130.6 mg/100 g d.w.), whereas kaempferol (159.1 mg/100 g d.w.), quercetin (119.6 mg/100 g d.w.), and sinapic acid (73.8 mg/100 g d.w.) dominated among phenolics. The lowest dose of kale leaves augmented the activity of catalase by 34% in males and by 44% in females. The highest applied dose increased activities of glutathione reductase by 31%, superoxide dismutase by 27%, and glutathione S-transferase by 24% in males only. Kale administration did not affect the activities of glutathione peroxidase and paraoxonase 1, glutathione concentration, lipid peroxidation level, and the level of DNA damage in the liver and the whole blood leukocytes. TEAC increased upon all doses of kale leaves by 30–90% in males and by 40–90% in females. The diet with kale did not affect blood biochemical and hematological markers. Results confirm no adverse effects of dried kale leaves used in model rat’s diet up to 60 g/kg for 90 days. The changes in hepatic antioxidant enzymes and plasma TEAC suggest beneficial effect of kale leaves on the antioxidant status in rats. Interestingly, these changes were more pronounced in male versus female animals.
This work was supported by the UE Project POIG 01.01.02-00-061/09 Bioactive Food.
The authors declare that they have no conflict of interest.
Aly, N., El-Gendy, K., Mahmoud, F., El-Sebae, A.K. (2010). Protective effect of vitamin C against chlorpyrifos oxidative stress in male mice. Pesticide Biochemistry and Physiology, 97(1), 7–12. https://doi. org/10.1016/j.pestbp.2009.11.007.
Anderson, D., Dobrzyńska, M., Yu, T.W. (1997). Modulating effects of silymarin and myricetin on food mutagens and doxorubicin in assays with different genetic endpoints. Journal of Environmental Pathology, Toxicology and Oncology, 16, 313–327.
Aronson, J.K. (2017). Defining ‘nutraceuticals’: neither nutritious nor pharmaceutical: defining ‘nutraceuticals.’ British Journal of Clinical Pharmacology, 83(1), SI, 8–19.
Boušová, I., Skálová, L. (2012). Inhibition and induction of glutathione S-transferases by flavonoids: possible pharmacological and toxicological consequences. Drug Metabolism Reviews, 44(4), 267–286.
Breinholt, V., Lauridsen, S.T., Dragsted, L.O. (1999). Differential effects of dietary flavonoids on drug metabolizing and antioxidant enzymes in female rat. Xenobiotica, 29(12), 1227–1240.
Cheng, A.C., Shen, C.J., Hung, C.M., Hsu, Y.C. (2019). Sulforaphane decrease of SERTAD1 expression triggers G1/S arrest in breast cancer cells. Journal of Medicinal Food, 22(5), 444–450.
Cieślik, E., Leszczyńska, T., Filipiak-Florkiewicz, A., Sikora, E., Pisulewski, P.M. (2007). Effects of some technological processes on glucosinolate contents in cruciferous vegetables. Food Chemistry, 105(3), 976–981.
Fuentes, F., Paredes-Gonzalez, X., Kong, A.N.T. (2015). Dietary glucosinolates sulforaphane, phenethyl isothiocyanate, indole-3-carbinol/3,3′-diindolylmethane: antioxidative stress/inflammation, Nrf2, epigenetics/epigenomics and in vivo cancer chemopreventive efficacy. Current Pharmacology Reports, 1, 179–196.
Galvez, J., Cruz, J.P. de la, Zarzuelo, A., Cuesta, F.S. de la (1995). Flavonoid inhibition of enzymic and nonenzymic lipid peroxidation in rat liver differs from its influence on the glutathione-related enzymes. Pharmacology, 51(2), 127–133.
Gul, S., Ahmed, S., Gul, H., Shad, K.F., Zia-Ul-Haq, M., Badiu, D. (2013). The antioxidant potential of Brassica rapa L. on glutathione peroxidase, superoxide dismutase enzymes and total antioxidant status. Revista Romana de Medicina de Laborator, 21(2), 161–169.
Güller, P., Karaman, M., Güller, U., Aksoy, M., Küfrevioğlu, Ö.İ. (2021). A study on the effects of inhibition mechanism of curcumin, quercetin, and resveratrol on human glutathione reductase through in vitro and in silico approaches. Journal of Biomolecular Structure and Dynamics, 39(5), 1744–1753.
ISO 9167-1:1992 Rapeseed and rapeseed meals – Determination of glucosinolates content – Method using high-performance liquid chromatography. Available online: (accessed on 28 February 2022).
James, D., Devaraj, S., Bellur, P., Lakkanna, S., Vicini, J., Boddupalli, S. (2012). Novel concepts of broccoli sulforaphanes and disease: induction of phase Ii antioxidant and detoxification enzymes by enhanced-glucoraphanin broccoli. Nutrition Reviews, 70(11), 654–665.
Jodynis-Liebert, J., Adamska, T., Ewertowska, M., Bylka, W., Matławska, I. (2009). Aquilegia vulgaris extract attenuates carbon tetrachloride-induced liver fibrosis in rats. Experimental and Toxicologic Pathology, 61(5), 443-451. https://doi: 10.1016/j.etp.2008.10.007.
Katalinic, V., Modun, D., Music, I., Boban, M. (2005). Gender differences in antioxidant capacity of rat tissues determined by 2,2′-azinobis (3-ethylbenzothiazoline 6-sulfonate; ABTS) and ferric reducing antioxidant power (FRAP) assays. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 140(1), 47–52.
Korus, A., Lisiewska, Z. (2011). Effect of preliminary processing and method of preservation on the content of selected antioxidative compounds in kale (Brassica oleracea L. var. acephala) leaves. Food Chemistry, 129(1), 149–154.
Korus, A., Słupski, J., Gebczyński, P., Banaś, A. (2014). Effect of preliminary processing and method of preservation on the content of glucosinolates in kale (Brassica oleracea L. var. acephala) leaves. LWT – Food Science and Technology, 59(2), Part 1, 1003–1008.
Krajka-Kuźniak, V., Szaefer, H., Bartoszek, A., Baer-Dubowska, W. (2011). Modulation of rat hepatic and kidney phase II enzymes by cabbage juices: comparison with the effects of indole-3-carbinol and phenethyl isothiocyanate. British Journal of Nutrition, 105(6), 816–826.
Kumru, S., Aydin, S., Gursu, M.F., Ozcan, Z., 2004. Changes of serum paraoxonase (an HDL-cholesterol-associated lipophilic antioxidant) and arylesterase activities in severe preeclamptic women. European Journal of Obstetrics and Gynecology and Reproductive Biology, 114(2), 177–181.
Kurilich, A.C., Tsau, G.J., Brown, A., Howard, L., Klein, B.P., Jeffery, E.H., Kushad, M., Wallig, M.A., Juvik, J.A. (1999). Carotene, tocopherol, and ascorbate contents in subspecies of Brassica oleracea. Journal of Agricultural and Food Chemistry, 47(4), 1576–1581.
Lou-Bonafonte, J.M., Gabás-Rivera, C., Navarro, M.A., Osada, J. (2015). PON1 and mediterranean diet. Nutrients, 7(6), 4068–4092.
Massafra, C., Gioia, D., De Felice, C., Picciolini, E., De Leo, V., Bonifazi, M., Bernabei, A. (2000). Effects of estrogens and androgens on erythrocyte antioxidant superoxide dismutase, catalase and glutathione peroxidase activities during the menstrual cycle. Journal of Endocrinology, 167(3), 447–452.
Melega, S., Canistro, D., Pagnotta, E., Iori, R., Sapone, A., Paolini, M. (2013). Effect of sprout extract from Tuscan black cabbage on xenobiotic-metabolizing and antioxidant enzymes in rat liver. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 751(1), 45–51.
Møller, P., Loft, S., Alfthan, G., Freese, R. (2004). Oxidative DNA damage in circulating mononuclear blood cells after ingestion of blackcurrant juice or anthocyanin-rich drink. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Nutrition and Carcinogenesis, 551(1-2), 119–126.
Phan, M.A.T., Paterson, J., Bucknall, M., Arcot, J. (2018). Interactions between phytochemicals from fruits and vegetables: Effects on bioactivities and bioavailability. Critical Reviews in Food Science and Nutrition, 58(8), 1310–1329.
Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. (2017). Oxidative stress: harms and benefits for human health. Oxidative Medicine and Cellular Longevity, 2017, 8416763.
Qu, G., Chen, J., Guo, X. (2018). The beneficial and deleterious role of dietary polyphenols on chronic degenerative diseases by regulating gene expression. BioScience Trends, 12(6), 526-536.
Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231–1237.
Sakr, S.A., Zoil, M.E., El-shafey, S.S. (2013). Ameliorative effect of grapefruit juice on amiodarone–induced cytogenetic and testicular damage in albino rats. Asian Pacific Journal of Tropical Biomedicine, 3(7), 573–579.
Šamec, D., Urlić, B., Salopek-Sondi, B. (2019). Kale (Brassica oleracea var. acephala) as a superfood: review of the scientific evidence behind the statement. Critical Reviews in Food Science and Nutrition, 59(15).
Sarıkamış, G., Balkaya, A., Yanmaz, R. (2008). Glucosinolates in kale genotypes from the Blacksea region of Turkey. Biotechnology and Biotechnological Equipment, 22(4), 942-946.
Sasaki, K., Neyazaki, M., Shindo, K., Ogawa, T., Momose, M. (2012). Quantitative profiling of glucosinolates by LC-MS analysis reveals several cultivars of cabbage and kale as promising sources of sulforaphane. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 903, 171–176.
Satheesh, N., Workneh Fanta, S. (2020). Kale: Review on nutritional composition, bio-active compounds, anti-nutritional factors, health beneficial properties and value-added products. Cogent Food and Agriculture, 6(1), art. no. 1811048.
Schmidt, S., Zietz, M., Schreiner, M., Rohn, S, Kroh, L.W. Krumbein, A. (2010). Identification of complex, naturally occurring flavonoid glycosides in kale (Brassica oleracea var. sabellica) by high-performance liquid chromatography diode-array detection/electrospray ionization multi-stage mass spectrometry. Rapid Communications. Mass Spectrometry, 24(14), 2009–2022.
Soundararajan, P., Kim, J.S. (2018). Anti-carcinogenic glucosinolates in cruciferous vegetables and their antagonistic effects on prevention of cancers. Molecules, 23(11), art. no. 2983.
Tiidus, P.M., Bombardier, E., Hidiroglou, N., Madere, R. (1999). Gender and exercise influence on tissue antioxidant vitamin status in rats. Journal of Nutritional Science and Vitaminology, 45(6), 701–710.
Tsao, R., Yang, R. (2003). Optimization of a new mobile phase to know the complex and real polyphenolic composition: towards a total phenolic index using high-performance liquid chromatography. Journal of Chromatography A, 1018(1), 29–40.
Vallejo, F., Tomás-Barberán, F.A., García-Viguera, C. (2002). Potential bioactive compounds in health promotion from broccoli cultivars grown in Spain. Journal of the Science of Food and Agriculture, 82(11), 1293–1297.
Vang, O., Rasmussen, B.F., Sørensen, H., Clausen, J., Andersen, O. (1995). Effects of dietary broccoli on antioxidant enzymes. Clinical Chemistry, 41(12), 1910–1911.
Velasco, P., Cartea, M.E., Gonzalez, C., Vilar, M., Ordas, A. (2007). Factors affecting the glucosinolate content of kale (Brassica oleracea acephala Group). Journal of Agricultural and Food Chemistry, 55(3), 955–962.
Verkerk, R., Dekker, M., Jongen, W.M.F. (2001). Post-harvest increase of indolyl glucosinolates in response to chopping and storage of Brassica vegetables. Journal of the Science of Food and Agriculture, 81(9), SI, 953-958.
Wiegand, H., Boesch-Saadatmandi, C., Regos, I., Treutter, D., Wolffram, S., Rimbach, G. (2009). Effects of quercetin and catechin on hepatic glutathione-S transferase (GST), NAD(P)H quinone oxidoreductase 1 (NQO1), and antioxidant enzyme activity levels in rats. Nutrition and Cancer, 61(5), 717–722.
Yagishita, Y., Fahey, J.W., Dinkova-Kostova, A.T., Kensler, T.W. (2019). Broccoli or sulforaphane: is it the source or dose that matters? Molecules, 24(19), art. no. 3593.
Yamamoto, T., Ohkuwa, T., Itoh, H., Sato, Y., Naoi, M. (2002). Effect of gender differences and voluntary exercise on antioxidant capacity in rats. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, 132(4), 437–444.
Zhang, Y.-J., Gan, R.Y., Li, S., Zhou, Y., Li, A.N., Xu, D.P., Li, H.B. (2015). Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules, 20(12), 21138–21156.
Zhu, C.Y., Loft, S. (2001). Effects of Brussels sprouts extracts on hydrogen peroxide-induced DNA strand breaks in human lymphocytes. Food and Chemical Toxicology, 39(12), 1191–1197.
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