ISOLATION AND IDENTIFICATION OF BIFIDOBACTERIA FROM INFANT GUT
 
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Pol. J. Food Nutr. Sci. 2003;53(Special issue 1s):90–94
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ABSTRACT
The research comprises isolation and identification of Bifidobacterium strains from faeces of breast-fed infants. Modified Garche’s agar medium, containing selective (penicillin and lithium chloride) or stimulating substances (maltodextrine instead of lactose), and anaerobic culture conditions (Gas Pak H2 + CO2, Oxoid) were used for strain isolation. Identification of isolates to the genus was based on fructose-6-phosphate phosphoketolase activity, lack of ability to produce catalase, indol from tryptofan, gas from glucose, and to reduce nitrate. Species identification was performed using PCR technique applying 16S rDNA-gene-targeted primers specific to B. infantis, B. breve and B. longum. Type strains from American Type Culture Collection (ATCC) were used as reference. As a result of the studies, 14 Bifidobacterium strains were isolated, among which 7 were classified to B. breve and 6 to B. longum species. B. infantis was not isolated. It was also impossible to identify Bifidobacterium sp. N10 in the applied system of primers.
ISSN:1230-0322