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1/2018 vol. 68
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Antioxidative Effects of Phenolic Compounds of Mushroom Mycelia in Simulated Regions of the Human Colon, In Vitro Study
Emanuel Vamanu
,
 
Diana Pelinescu
,
 
Ionela Avram
 
 
 
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Publication date: 2018-03-31
 
 
Pol. J. Food Nutr. Sci. 2018;68(1):83-90
DOI: https://doi.org/10.1515/pjfns-2017-0010
 
 
Article (PDF)
References (43) Citations (7)
 
KEYWORDS
Antioxidant
gentisic acid
GIS1 system
microbiota
mycelia
Pleurotus ostreatus
 
ABSTRACT
Many compounds in mushrooms are biologically active; however, the in vivo actions of their metabolites are poorly understood. An in vitro system, GIS1, was used to simulate the fermentation action of microbiota in each colon region. We used MycoPo, a natural product obtained from the lyophilized mycelia of different Pleurotus ostreatus species to determine the biological effects in human-colon regions. Controls (Lentinula edodes mycelia; dried basidia of Agaricus brunnescens) were chosen to confirm the biological activity of P. ostreatus mycelia in vitro. We measured total antioxidant capacity and ferric ion-reducing antioxidant power (FRAP) in simulated colon regions to identify antioxidant compounds, and undertook in vitro gastrointestinal simulation and microbiological analyses. The highest FRAP was found for the ascending colon, and the antioxidant effect was higher when MycoPo was administered. A. brunnescens consumption resulted in low total antioxidant capacity. Polyphenol content was correlated with the antioxidant status and microbial composition of microbiota. Total polyphenolic content was higher after A. brunnescens consumption, and four types of polyphenols were identified by high-performance liquid chromatography. Major phenolic acids were gentisic acid, homogentisic acid, and small amounts of caffeic acid. The Enterobacteriaceae species populations varied greatly across the three parts of the colon. We noted a significant (p<0.01) correlation between antioxidant status in the transverse and descending colon after MycoPo administration, and A. brunnescens consumption with the number of Lactobacillus and Bifidobacteria species (R2>0.85). These data suggest a direct relationship between favorable bacterial strains and availability of bioactive compounds, with specificity for each colon region.
REFERENCES (43)
1.
Alpinar K., Ozyurek M., Kolak U., Guclu K., Aras C., Altun M., Celik S.E., Berker K.I., Bektasoglu B., Apak R., Antioxidant capacities of some food plants wildly grown in Ayvalik of Turkey. Food Sci. Technol. Res., 2009, 15, 59-64.
Google Scholar
 
 
2.
Bartosch S., Fite A., Macfarlane G.T., McMurdo E.T., Characterization of bacterial communities in feces from healthy elderly volunteers and hospitalized elderly patients by using real-time PCR and effects of antibiotic treatment on the fecal microbiota. Appl. Environ. Microbiol., 2004, 70, 3575-3581.
Google Scholar
 
 
3.
Chen S.Y., Ho K.J., Liang C.H., Tsai C.H., Huang L.Y., Mau J.L., Preparation of culinary-medicinal king oyster mushroom Pleurotus eryngii-fermented products with high ergothioneine content and their taste quality. Int. J. Med. Mush., 2012, 14, 85-93.
Google Scholar
 
 
4.
Choi C.H., Chang S.K., Alteration of gut microbiota and efficacy of probiotics in functional constipation. J. Neurogastroenterol. Motil., 2015, 21, 4-7.
Google Scholar
 
 
5.
Conlon M.A., Bird A.R., The impact of diet and lifestyle on gut microbiota and human health. Nutrients, 2015, 7, 17-44.
Google Scholar
 
 
6.
Din A.R.J.M., Razak S.A., Sabaratnam V., Effect of mushroom supplementation as a prebiotic compound in super worm based diet on growth performance of red tilapia fingerlings. Sains Malaysiana, 2012, 41, 1197-1203.
Google Scholar
 
 
7.
Diwan R., Shinde A., Malpathak N., Phytochemical composition and antioxidant potential of Ruta graveolens L. in vitro culture lines. J. Bot., 2012, Article ID 685427.
Google Scholar
 
 
8.
Ekor M., The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front. Pharmacol., 2014, 4, 177.
Google Scholar
 
 
9.
Elisashvili V., Submerged cultivation of medicinal mushrooms: bioprocesses and products (review). Int. J. Med. Mush., 2012, 14, 211-239.
Google Scholar
 
 
10.
Green R.J., Murphy A.S., Schulz B., Watkins B.A., Ferruzzi M.G., Common tea formulations modulate in vitro digestive recovery of green tea catechins. Mol. Nutr. Food Res., 2007, 51, 1152-1162.
Google Scholar
 
 
11.
Guerra A., Etienne-Mesmin L., Livrelli V., Denis S., Blanquet-Diot S., Alric M., Relevance and challenges in modeling human gastric and small intestinal digestion. Trends Biotechnol., 2012, 30, 591-600.
Google Scholar
 
 
12.
Gerritsen J., Smidt H., Rijkers G.T., de Vos W.M., Intestinal microbiota in human health and disease: the impact of probiotics. Genes Nutr., 2011, 6, 209-240.
Google Scholar
 
 
13.
Hamer H.M., Jonkers D., Venema K., Vanhoutvin S., Troost F.J., Brummer R.J., Review article: the role of butyrate on colonic function. Aliment. Pharmacol. Therapeut., 2008, 27, 104-119.
Google Scholar
 
 
14.
Heilig H.G., Zoetendal E.G., Vaughan E.E., Marteau P., Akkermans A.D., de Vos W.M., Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl. Environ. Microbiol., 2002, 68, 114-123.
Google Scholar
 
 
15.
Hughes R., Magee E.A.M., Bingham S., Protein degradation in the large intestine: relevance to colorectal cancer. Curr. Issues Intest. Microbiol., 2000, 1, 51-58.
Google Scholar
 
 
16.
Hur S.J., Lim B.O., Decker E.A., McClements D.J., In vitro human digestion models for food applications. Food Chem., 2011, 125, 1-12.
Google Scholar
 
 
17.
Hutcheson R., Rocic P., The metabolic syndrome, oxidative stress, environment, and cardiovascular disease: the great exploration. Exp. Diabetes Res., 2012, Article ID 271028.
Google Scholar
 
 
18.
Jeong S.C., Koyyalamudi S.R., Hughes J., Khoo C., Bailey T., Marripudi K., Park J.P., Kim J.H., Song C.H., Antioxidant and immunomodulating activities of exo- and endopolysaccharide fractions from submerged mycelia cultures of culinary-medicinal mushrooms. Int. J. Med. Mush., 2013, 15, 251-266.
Google Scholar
 
 
19.
Kailasapathy K., Chin, J., Survival and therapeutic potential of probiotic organisms with reference to Lactobacillus acidophilus and Bifidobacterium spp.. Immun. Cell Biol., 2000, 78, 80-88.
Google Scholar
 
 
20.
Koleva P.T., Bridgman S.L., Kozyrskyj A.L., The infant gut microbiome: evidence for obesity risk and dietary intervention. Nutrients, 2015, 7, 2237-2260.
Google Scholar
 
 
21.
Lam Y.S., Okello E.J., Determination of lovastatin, β-glucan, total polyphenols, and antioxidant activity in raw and processed oyster culinary-medicinal mushroom, Pleurotus ostreatus (higher Basidiomycetes). Int. J. Med. Mush., 2015, 17, 117-128.
Google Scholar
 
 
22.
Lee B.C., Bae J.T., Pyo H.B., Choe T.B., Sang W.K., Hwang H.J., Yun J.W., Submerged culture conditions for the production of mycelial biomass and exopolysaccharides by the edible Basidiomycete Grifola frondosa. Enz. Microbial Technol., 2004, 35, 369-376.
Google Scholar
 
 
23.
Lindequist U., Niedermeyer T.H.J., Jülich W.D., The pharmacological potential of mushrooms. Evid. Based Complement Alt. Med., 2005, 2, 285-299.
Google Scholar
 
 
24.
Mai V., Torrazza R.M., Ukhanova M., Wang X., Sun Y., Li N., Shuster J., Sharma R., Hudak M.L., Neu J., Distortions in development of intestinal microbiota associated with late onset sepsis in preterm infants. PLOS One, 2013, 8, e52876.
Google Scholar
 
 
25.
Manach C., Scalbert A., Morand C., Remesy C., Jimenez L., Polyphenols: food sources and bioavailability. Am. J. Clin. Nutr., 2004, 79, 5727-5747.
Google Scholar
 
 
26.
Mitsuyama K., Sata M., Gut microflora: a new target for therapeutic approaches in inflammatory bowel disease. Expert Opin. Ther. Tar., 2008, 12, 301-312.
Google Scholar
 
 
27.
Ozyurek M., Bener M., Guclu K., Donmez A.A., Suzgec-Selcuk S., Pirildar S., Mericli A.H., Apak R., Evaluation of antioxidant activity of Crataegus species collected from different regions of Turkey. Rec. Nat. Prod., 2012, 6, 263-277.
Google Scholar
 
 
28.
Prasad R., Varshney V.K., Harsh N.S., Kumar M., Antioxidant capacity and total phenolics content of the fruiting bodies and submerged cultured mycelia of sixteen higher Basidiomycetes mushrooms from India. Int. J. Med. Mush., 2015, 17, 933-941.
Google Scholar
 
 
29.
Premanath R., Sudisha J., Devi L.N., Aradhya S.M.. Antibacterial and anti-oxidant activities of fenugreek (Trigonella foenum graecum L.) leaves. J. Med. Plants Res., 2011, 5, 695-705 [http://docsdrive.com/pdfs/acad...].
Google Scholar
 
 
30.
Qin X., Emerson J., Stapp J., Stapp L., Abe, P., Burns J.L., Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J. Clin. Microbiol., 2003, 41, 4312-4317.
Google Scholar
 
 
31.
Rinttila T., Kassinen A., Malinen E., Krogius L., Palva A., Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J. Appl. Microbiol., 2004, 97, 1166-1177.
Google Scholar
 
 
32.
Rodriguez-Roque M.J., Rojas-Grau M.A., Elez-Martinez P., Martin-Belloso O., Soymilk phenolic compounds, isoflavones and antioxidant activity as affected by in vitro gastrointestinal digestion. Food Chem., 2013, 136, 206-212.
Google Scholar
 
 
33.
Rossi M., Corradini C., Amaretti A., Nicolini M., Pompei A., Zanoni S., Matteuzzi D., Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures. Appl. Environ. Microbiol., 2005, 71, 6150-6158.
Google Scholar
 
 
34.
Synytsya A., Mickova K., Synytsya A., Jablonsky I., Spevacek J., Erban V., Kovarikova E., Copikova J., Glucans from fruit bodies of cultivated mushrooms Pleurotus ostreatus and Pleurotus eryngii: structure and potential prebiotic activity. Carbohydr. Pol., 2009, 76, 548–556.
Google Scholar
 
 
35.
Tzortzis G., Goulas A.K., Gee J.M., Gibson G.R., Novel galactooligosaccharide mixture increases the bifidobacterial population numbers in a continuous in vitro fermentation system and in the proximal colonic contents of pigs in vivo. J. Nutr., 2005, 135, 1726-1731.
Google Scholar
 
 
36.
Vamanu E., Biological activities of the polysaccharides produced in submerged culture of two edible Pleurotus ostreatus mushrooms. J. Biomed. Biotechnol., 2012, Article ID 565974.
Google Scholar
 
 
37.
Vamanu E., Nita S., Biological activity of fluidized bed ethanol extracts from several edible mushrooms. Food Sci. Biotechnol., 2014, 23, 1483-1490.
Google Scholar
 
 
38.
Vamanu E., Pelinescu D., Avram I., Niță S., Vamanu A., Study of PROBAC product influence on infant microbiota in a single chamber colonic fermentation model GIS1. Ann. Microbiol., 2012, 63, 1029-1038.
Google Scholar
 
 
39.
Vamanu E., Pelinescu D., Avram I., Nita S., An in-vitro evaluation of antioxidant and colonic microbial profile levels following mushroom consumption. BioMed Res. Int., 2013, Article ID 289821.
Google Scholar
 
 
40.
van de Heijning B.J.M., Berton A., Bouritius H., Goulet O., GI symptoms in infants are a potential target for fermented infant milk formulae: a review. Nutrients, 2014, 6, 3942-3967.
Google Scholar
 
 
41.
Wasser S.P., Medicinal mushroom science: Current perspectives, advances, evidences, and challenges. Biomed. J., 2014, 37, 345-356.
Google Scholar
 
 
42.
Wang Y., Ametaj B.N., Ambrose D.J., Gänzle M.G., Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. BMC Microbiol., 2013, 13, 19.
Google Scholar
 
 
43.
Yokota M.E., Frison P.S., Marcante R.C., Jorge L.F., Valle J.S., Dragunski D.C., Colauto N.B., Linde G.A., Iron translocation in Pleurotus ostreatus basidiocarps: production, bioavailability, and antioxidant activity. Gen. Mol. Res., 2016, 15, Art. no. UNSP 1501788.
Google Scholar
 
 
 
CITATIONS (7):
1.
Altered in Vitro Metabolomic Response of the Human Microbiota to Sweeteners
Emanuel Vamanu, Diana Pelinescu, Florentina Gatea, Ionela Sârbu
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2.
Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms
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Evidence-Based Complementary and Alternative Medicine
 
CrossRef
 
 
3.
Antioxidant, anti-inflammatory and cytotoxic/antitumoral bioactives from the phylum Basidiomycota and their possible mechanisms of action
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Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach
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5.
Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds
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6.
Extrapolating Qualitative Mycochemical Profiling and Therapeutic Potential of Two Mycorrhizal Amanita Species (Agaricomycetes) from Pakistan
Muniba Shafique, Abdul Niazi, Muhammad Imran, Muhammad Zaheer
International Journal of Medicinal Mushrooms
 
CrossRef
 
 
7.
Harnessing antifungal metabolites from macro basidiomycetes against wilt inciting Fusarium spp.
B. S., S. A., Nakkeeran S., Sivakumar U., Thiribhuvanamala G.
Journal of Biological Control
 
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