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1/2018 vol. 68
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Antioxidative Effects of Phenolic Compounds of Mushroom Mycelia in Simulated Regions of the Human Colon, In Vitro Study
Emanuel Vamanu
,
 
Diana Pelinescu
,
 
Ionela Avram
 
 
 
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Publication date: 2018-03-31
 
 
Pol. J. Food Nutr. Sci. 2018;68(1):83-90
DOI: https://doi.org/10.1515/pjfns-2017-0010
 
 
Article (PDF)
References (43) Citations (7)
 
KEYWORDS
Antioxidant
gentisic acid
GIS1 system
microbiota
mycelia
Pleurotus ostreatus
 
ABSTRACT
Many compounds in mushrooms are biologically active; however, the in vivo actions of their metabolites are poorly understood. An in vitro system, GIS1, was used to simulate the fermentation action of microbiota in each colon region. We used MycoPo, a natural product obtained from the lyophilized mycelia of different Pleurotus ostreatus species to determine the biological effects in human-colon regions. Controls (Lentinula edodes mycelia; dried basidia of Agaricus brunnescens) were chosen to confirm the biological activity of P. ostreatus mycelia in vitro. We measured total antioxidant capacity and ferric ion-reducing antioxidant power (FRAP) in simulated colon regions to identify antioxidant compounds, and undertook in vitro gastrointestinal simulation and microbiological analyses. The highest FRAP was found for the ascending colon, and the antioxidant effect was higher when MycoPo was administered. A. brunnescens consumption resulted in low total antioxidant capacity. Polyphenol content was correlated with the antioxidant status and microbial composition of microbiota. Total polyphenolic content was higher after A. brunnescens consumption, and four types of polyphenols were identified by high-performance liquid chromatography. Major phenolic acids were gentisic acid, homogentisic acid, and small amounts of caffeic acid. The Enterobacteriaceae species populations varied greatly across the three parts of the colon. We noted a significant (p<0.01) correlation between antioxidant status in the transverse and descending colon after MycoPo administration, and A. brunnescens consumption with the number of Lactobacillus and Bifidobacteria species (R2>0.85). These data suggest a direct relationship between favorable bacterial strains and availability of bioactive compounds, with specificity for each colon region.
 
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Altered in Vitro Metabolomic Response of the Human Microbiota to Sweeteners
Emanuel Vamanu, Diana Pelinescu, Florentina Gatea, Ionela Sârbu
Genes
 
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2.
Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms
Gebreselema Gebreyohannes, Andrew Nyerere, Christine Bii, Sbhatu Berhe
Evidence-Based Complementary and Alternative Medicine
 
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Antioxidant, anti-inflammatory and cytotoxic/antitumoral bioactives from the phylum Basidiomycota and their possible mechanisms of action
Gomes Vieira, Alencar de, Reis dos, Lima de, Oliveira de, Mata da, Dias Soares, Costa da, Medeiros de, Márcia Paz, e Gayoso, e Castro, Muhammad Islam, Cavalcante Melo
Biomedicine & Pharmacotherapy
 
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4.
Validating Anti-Infective Activity of Pleurotus Opuntiae via Standardization of Its Bioactive Mycoconstituents through Multimodal Biochemical Approach
Pandey Tiwari, Ishan Pandey, Anurag Kanase, Amita Verma, Beatriz Garcia-Canibano, Sarada Dakua, Shidin Balakrishnan, Mohan Singh
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Molecular identification and antimicrobial activities of some wild Egyptian mushrooms: Bjerkandera adusta as a promising source of bioactive antimicrobial phenolic compounds
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Journal of Genetic Engineering and Biotechnology
 
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Extrapolating Qualitative Mycochemical Profiling and Therapeutic Potential of Two Mycorrhizal Amanita Species (Agaricomycetes) from Pakistan
Muniba Shafique, Abdul Niazi, Muhammad Imran, Muhammad Zaheer
International Journal of Medicinal Mushrooms
 
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Harnessing antifungal metabolites from macro basidiomycetes against wilt inciting Fusarium spp.
B. S., S. A., Nakkeeran S., Sivakumar U., Thiribhuvanamala G.
Journal of Biological Control
 
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