BIOMASS OF CANDIDA UTILIS ATCC 9950 YEAST AS A SOURCE OF MAGNESIUM BIOPLEXES
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Publication date: 2006-03-31
Pol. J. Food Nutr. Sci. 2006;56(1):9-16
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ABSTRACT
The study was aimed at investigating the possibility of obtaining a natural bioplex of magnesium from the biomass of fodder yeast C.utilis ATCC 9950 enriched in that element. Yeast cultures were run under dynamic conditions (with aeration) for 48 h on a control medium (YPD) and the experimental one enriched in magnesium ions at a concentration of 1.25 g/L. During the logarithmic phase of growth, magnesium was observed to bind the mannoprotein layer of the cell wall of yeast (68% of the total magnesium in biomass), whereas in the stationary phase it was subject to intracellular bioaccumulation. Consequently, 61% of the cellular magnesium pool were accumulated in protoplasts and 39% in cell walls. The bioplex separated from the 48-h experimental culture contained nearly 3-fold more magnesium (8.51 mg Mg/g d.s.) than that isolated from the control culture (2.92 mg Mg/g d.s.).