Rats of control group were fed a standard diet (LSM). One of the diets was supplemented with 1% cholesterol (LSM+CH), another one with 5% freeze-dried apple pulp (APP), and the last one with both, 1% cholesterol and 5% apple pulp. Dietary addition of apple pulp significantly increased calcium concentration in femur bone from 154.2±5.6 (LSM, LSM+CH) to 180.1±4.8 g/kg wet weight (APP, APP+CH), while bone magnesium content was reduced from 3.34±0.14 to 2.70±0.12 g/kg. Dietary cholesterol (1%) had the similar significant effect on the bone content of these macroelements, and decreased bone manganese content. Moreover, rats fed diets with cholesterol (LSM+CH, APP+CH) revealed the higher Ca concentration in kidney and the lower liver Mg content (p<0.05) than in the other groups (LSM, APP). The 5% administration of the freeze-dried apple pulp reduced significantly kidney Mn content. It may be concluded that both dietary factors studied (apple pulp and cholesterol) positively influence calcification of bones but a high level of cholesterol significantly reduces bioavailability and/or storage of the other important macroelement – magnesium.