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ORIGINAL ARTICLE
Use of High-Protein Milk Preparations in the Production of Probiotic Fresh Cheeses
 
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Department of Dairy Science and Quality Management, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Oczapowskiego 7, 10–719 Olsztyn, Poland
 
2
Department of Commodity Science and Food Analysis, University of Warmia and Mazury in Olsztyn, 10-726 Olsztyn, Poland
 
 
Submission date: 2024-12-05
 
 
Acceptance date: 2025-03-28
 
 
Online publication date: 2025-04-17
 
 
Publication date: 2025-04-17
 
 
Corresponding author
Michał Smoczyński   

Department of Dairy Science and Quality Management, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Oczapowskiego 7, 10–719 Olsztyn, Poland
 
 
Pol. J. Food Nutr. Sci. 2025;75(2):119-134
 
KEYWORDS
TOPICS
ABSTRACT
The study on the use of skimmed milk and buttermilk separation products obtained by membrane filtration and the Lactobacillus acidophilus LA-5 probiotic culture in the production of fresh cheeses was undertaken. Membrane separation products – micellar casein concentrate (MMC), buttermilk protein concentrate (RMFB), a mixture of micellar casein concentrate and a buttermilk serum protein concentrate (RUFP) – were used in liquid and powder form. Fresh cheeses were produced from fluid protein concentrates or from milk with protein powder addition. A control sample was produced from milk with the addition of skimmed milk powder. Fresh cheeses produced from MCC were characterised by a desirable, high content of protein, calcium, and phosphorus. In turn, magnesium content was highest in fresh cheeses made from RUFP. In all cheeses, Lb. acidophilus LA-5 counts exceeded log 6 cfu/g on the last day of storage (day 21), thus satisfying the criteria for probiotic products. Fresh cheese made from MCC was characterised by the greatest difference in colour relative to the control sample. In addition, cheeses produced from MCC fluid or with the addition of MCC powder were characterised by higher firmness (69.58 and 41.67 N, respectively) relative to the cheese produced from RMFB (3.35–3.37 N) or RUFP (5.89–21.96 N). The power law model accurately predicted the rheological properties of the examined cheeses (R2>0.999). All cheeses displayed pseudoplastic flow behaviour, where the storage modulus (G') was higher than the loss modulus (G") and was not dependent on frequency. The fractal analysis revealed that MCC cheeses had the least irregular microstructure with the lowest values of fractal dimension. The use of high-protein preparations in the production of fresh cheeses generally decreased their sensory acceptability. It can be concluded that probiotic fresh cheeses made from skimmed milk and buttermilk separation products with the addition of the Lb. acidophilus LA-5 culture differ in physicochemical properties and sensory attributes.
FUNDING
The publication process was conducted within the project funded under the designated subsidy of the Minister of Science Republic of Poland; task entitled ‘The Research Network of Life Sciences Universities for the Development of the Polish Dairy Industry—Research Project’ (MEiN/2023/DPI/2875).
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper.
REFERENCES (50)
1.
Aljewicz M., Cichosz G. (2015). Protective effects of Lactobacillus cultures in Dutch-type cheese-like products. LWT – Food Science and Technology, 63(1), 52–56. https://doi.org/10.1016/j.lwt.2015.03.054
 
2.
Aljewicz M., Mulet-Cabero A.I., Wilde P.J. (2021). A comparative study of the influence of the content and source of β-glucan on the rheological, microstructural properties and stability of milk gel during acidification. Food Hydrocolloids, 113, art. no. 06486. https://doi.org/10.1016/j.foodhyd.2020.106486
 
3.
Aljewicz M., Polak-Juszczyk L., Juśkiewicz J. (2018). The impact of different structure of β-glucans and acidity of milk gel on the bioavailability of mineral compounds in growing rats. Journal of Functional Foods, 49, 214–223. https://doi.org/10.1016/j.jff.2018.07.063
 
4.
AOAC. (2007). Official Methods of Analysis of AOAC International (18th ed). The Association of Official Analytical Chemists International, Gaithersburg, MD, USA.
 
5.
Barrett A.H., Peleg M. (1995). Applications of fractal analysis to food structure. LWT – Food Science and Technology, 28(6), 553–563. https://doi.org/10.1016/0023-6438(95)90001-2
 
6.
Bolivar-Jacobo N.A., Reyes Villagrana R.A., Rentería-Monterrubio A.L., Sánchez-Vega R., Santellano-Estrada E., Tirado-Gallegos J.M., Chávez-Martínez A. (2023). Culture age, growth medium, ultrasound amplitude, and time of exposure influence the kinetic growth of Lactobacillus acidophilus. Fermentation, 9(1), art. no. 63. https://doi.org/10.3390/fermentation9010063
 
7.
Carter B.G., Cheng N., Kapoor R., Meletharayil G.H., Drake M.A. (2021). Invited review: Microfiltration-derived casein and whey proteins from milk. Journal of Dairy Science, 104(3), 2465–2479. https://doi.org/10.3168/jds.2020-18811
 
8.
Dec B., Kiełczewska K., Smoczyński M., Baranowska M., Kowalik J. (2023). Properties and fractal analysis of high-protein milk powders. Applied Sciences, 13(6), art. no. 3573. https://doi.org/10.3390/app13063573
 
9.
Dobrzańska A., Cais-Sokolińska D. (2014). Measuring the brightness and coordinate trichromaticity milk protein preparations. Aparatura Badawcza i Dydaktycza, 19(3), 267–272 (in Polish, English abstract).
 
10.
Dziuba J., Smoczyński M., Dziuba Z., Smoczyński L. (1997). A new fractal approach to the structure of casein gels. Milchwissenschaft, 52(8), 448–451.
 
11.
EN ISO 13299: 2016-05E. Sensory analysis. Methodology. General guidance for establishing a sensory profile.
 
12.
EN ISO 8586: 2014–03. Sensory analysis. General guidelines for the selection, training and monitoring of selected assessors and expert sensory assessors.
 
13.
Esteves C.L., Lucey J.A., Hyslop D.B., Pires E.M. (2003). Effect of gelation temperature on the properties of skim milk gels made from plant coagulants and chymosin. International Dairy Journal, 13(11), 877–885. https://doi.org/10.1016/S0958-6946(03)00114-6
 
14.
Evans J., Żulewska J., Newbold M., Drake M.A., Barbano D.M. (2009). Comparison of composition, sensory, and volatile components of 34% whey protein and milk serum protein concentrates. Journal of Dairy Science, 92(10), 4773–4791. https://doi.org/10.3168/jds.2009-2194
 
15.
Gaucheron F. (2005). The minerals of milk. Reproduction Nutrition Development, 45(4), 473–483. https://doi.org/10.1051/rnd:2005030
 
16.
Guneser O., Aydin B. (2022). Characterization of Quark-like probiotic cheese produced from a mixture of buffalo milk and cow milk. Mljekarstvo: Journal for Dairy Production and Processing Improvement, 72(3), 172–188. https://doi.org/10.15567/mljekarstvo.2022.0306
 
17.
Huppertz T. (2013). Chemistry of the caseins. In P.L.H. McSweeney, P.F. Fox (Eds.), Advanced Dairy Chemistry. Proteins: Basic Aspects, vol. 1A, 4th edition. Springer New York Heidelberg Dordrecht London, pp. 135–160. https://doi.org/10.1007/978-1-4614-4714-6_4
 
18.
ISO 8070: 2007. Milk and milk products — Determination of calcium, sodium, potassium and magnesium contents. Atomic absorption spectrometric method. International Dairy Federation, Brussels, Belgium.
 
19.
ISO/TS 17996:2006 [IDF/RM 205: 2006]. Cheese – Determination of rheological properties by uniaxial compression at constant displacement rate.
 
20.
Kadiya K.S., Kanawjia S.K., Solanki A.K. (2014). Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of quarg cheese. International Journal of Fermented Foods, 3(1), 61–76. https://doi.org/10.5958/2321-712X.2014.01309.X
 
21.
Kiełczewska K., Dąbrowska A., Bielecka M.M., Dec B., Baranowska M., Ziajka J., Zhennai Y., Zulewska J. (2022). Protein preparations as ingredients for the enrichment of non-fermented milks. Foods, 11(13), art. no. 1817. https://doi.org/10.3390/foods11131817
 
22.
Kommineni A., Sunkesula V., Marella C, Metzger L.E. (2022). Calcium-reduced micellar casein concentrate – Physicochemical properties of powders and functional properties of the dispersions. Foods, 11(10), art. no. 1377. https://doi.org/10.3390/foods11101377
 
23.
Kowalska M., Ambroziak A., Aljewicz M., Cichosz G. (2012). Fortification of dairy products by calcium and magnesium. Postępy Techniki Przetwórstwa Spożywczego, 22(1), 93–98 (in Polish, English abstract).
 
24.
Królczyk J., Dawidziuk T., Janiszewska-Turak E., Sołowiej B. (2016). Use of whey and whey preparations in the food industry – A review. Polish Journal of Food and Nutrition Sciences, 66(3), 157–165. https://doi.org/10.1515/pjfns-2015-0052
 
25.
Laemmli U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685. https://doi.org/10.1038/227680a0
 
26.
Lee Y.R., Hong Y.H. (2003). Electrophoretic behaviors of α-lactalbumin and β-lactoglobulin mixtures caused by heat treatment. Asian-Australasian Journal of Animal Sciences, 16(7), 1041–1045. https://doi.org/10.5713/ajas.2003.1041
 
27.
Lis A., Staniewski B., Ziajka J. (2021). A comparison of butter texture measurements with the AP 4/2 penetrometer and TA.XT. Plus texture analyzer. International Journal of Food Properties, 24(1), 1744–1757. https://doi.org/10.1080/10942912.2021.1999262
 
28.
Lucey J.A. (2002). Formation and physical properties of milk protein gels. Journal of Dairy Science, 85(2), 281–294. https://doi.org/10.3168/jds.S0022-0302(02)74078-2
 
29.
Lucey J.A., Singh H. (1997). Formation and physical properties of acid milk gels: A review. Food Research International, 30(7), 529–542. https://doi.org/10.1016/S0963-9969(98)00015-5
 
30.
Madadlou A., Khosroshahi A., Mousavi S.M., Djome Z.E. (2006). Microstructure and rheological properties of Iranian white cheese coagulated at various temperatures. Journal of Dairy Science, 89(7), 2359–2364. https://doi.org/10.3168/jds.S0022-0302(06)72308-6
 
31.
McMahon D.J., Oommen B.S. (2013). Casein micelle structure, functions, and interactions. In P.L.H. McSweeney, P.F. Fox (Eds.), Advanced Dairy Chemistry, Proteins: Basic Aspects, vol. 1A, 4th edition, Springer New York Heidelberg Dordrecht London, pp. 185–210. https://doi.org/10.1007/978-1-4614-4714-6_6
 
32.
McSweeney D.J., O’Mahony J.A., McCarthy N.A. (2021). Strategies to enhance the rehydration performance of micellar casein dominant dairy powders. International Dairy Journal, 122, art. no. 105116. https://doi.org/10.1016/j.idairyj.2021.105116
 
33.
Mezger T. (2012). Chapter 2 – Flow behavior and viscosity. In The Rheology Handbook: 4th Edition (pp. 21–32). Hannover, Germany: Vincentz Network. https://doi.org/10.1515/9783748600367-003
 
34.
Miocinovic J., Le Trung T., Fredrick E., Van der Meeren P., Pudja P., Dewettinck K. (2014). A comparison of composition and emulsifying properties of MFGM materials prepared from different dairy sources by microfiltration. Food Science and Technology International, 20(6), 441–451. https://doi.org/10.1177/1082013213489566
 
35.
Pathare P.B., Opara U.L., Al-Said F.A.-J. (2013). Colour measurement and analysis in fresh and processed foods: A review. Food and Bioprocess Technology, 6(1), 36–60. https://doi.org/10.1007/s11947-012-0867-9
 
36.
Pulliainen T.K., Wallin H.C. (1994). Determination of total phosphorus in foods by colorimetric measurement of phosphorus as molybdenum blue after dry-ashing: NMKL1 Interlaboratory Study. The Journal of AOAC International, 77(6), 1557–1561. https://doi.org/10.1093/jaoac/77.6.1557
 
37.
Saleh A., Mohamed A.A., Alamri M.S., Hussain S., Qasem A.A., Ibraheem M.A. (2020). Effect of different starches on the rheological, sensory and storage attributes of non-fat set yogurt. Foods, 9(1), art. no. 61. https://doi.org/10.3390/foods9010061
 
38.
Salunke P., Marella C., Metzger L.E. (2021). Microfiltration and ultrafiltration process to produce micellar casein and milk protein concentrates with 80% crude protein content: Partitioning of various protein fractions and constituents. Dairy, 2(3), 367–384. https://doi.org/10.3390/dairy2030029
 
39.
Simov J., Maubois J.L., Garem A., Camier B. (2005). Making of kashkaval cheese from bovine micellar casein powder. Le Lait, 85(6), 527–533. https://doi.org/10.1051/lait:2005035
 
40.
Smoczyński M. (2020). Fractal analysis of the microstructure of milk powders produced at various temperatures. Journal of Food Science and Technology, 57, 2303–2309. https://doi.org/10.1007/s13197-020-04268-x
 
41.
Smoczyński M., Baranowska M. (2014). A fractal approach to microstructural changes during the storage of yoghurts prepared with starter cultures producing exopolysaccharides. Journal of Texture Studies, 45(2), 121–129. https://doi.org/10.1111/jtxs.12055
 
42.
Soltanzadeh M., Hesari J., Peighambardoust S.H. (2019). Study of chemical and microbial properties of probiotic quark cheese containing Lactobacillus acidophilus and Lactobacillus casei. Iranian Journal of Biosystems Engineering, 50(2), 375–388. https://doi.org/10.22059/ijbse.2019.270715.665124
 
43.
Spitsberg V.L. (2005). Invited review: Bovine milk fat globule membrane as a potential nutraceutical. Journal of Dairy Science, 88(7), 2289–2294. https://doi.org/10.3168/jds.S0022-0302(05)72906-4
 
44.
Suthar J., Jana A., Balakrishnan S. (2017). High protein milk ingredients – a tool for value-addition to dairy and food products. Journal of Dairy, Veterinary & Animal Research, 6(1), art. no. 00171. https://doi.org/10.15406/jdvar.2017.06.00171
 
45.
Szajewska H., Berni Canani R., Domellöf M., Guarino A., Hojsak I., Indrio F., Lo Vecchio A., Mihatsch W., Mosca A., Orel R., Salvatore S., Shamir R., van den Akker C.H.P., van Goudoever J.B., Vandenplas Y., Weizman Z. (2023). Probiotics for the management of peadiatric gastrointestinal disorders: position paper of the ESPGHAN special interest group on gut microbiota and modification. Journal of Pediatric Gastroenterology and Nutrition, 76(2), 232–247. https://doi.org/10.1097/MPG.0000000000003633
 
46.
Wium H., Qvist K. (1998). Effect of rennet concentration and method of coagulation on the texture of Feta cheeses made from ultrafiltered bovine milk. Journal of Dairy Research, 65(4), 653–663. https://doi:10.1017/S0022029998003094
 
47.
Wojtasik A., Woźniak A., Stoś K., Jarosz M. (2020). Mineral ingredients. In M. Jarosz, E. Rychlik, K. Stoś, J. Charzewska (Eds.), Nutrition Standards for the Polish Population and Their Application, Narodowy Instytut Zdrowia Publicznego – Państwowy Zakład Higieny, Warszawa, pp. 273–315 (in Polish).
 
48.
Zhang M., Sun X., Cheng J., Guo M. (2022). Analysis and comparison of nutrition profiles of canine milk with bovine and caprine milk. Foods, 11(3), art. no. 472. https://doi.org/10.3390/foods11030472
 
49.
Zhu C., Brown C., Gillies G., Watkinson P., Bronlund J. (2015). Characterizing the rheological properties of mozzarella cheese at shear rate and temperature conditions relevant to pizza baking. LWT – Food Science and Technology, 64(1), 82–87. https://doi.org/10.1016/j.lwt.2015.05.037
 
50.
Żulewska J., Tarapata J., Dec B., Baranowska M. (2024). Sposób wytwarzania wysokobiałkowego preparatu z mleka i maślanki. Patent nr. P.450275 [WIPO ST 10/C PL450275].
 
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