BIOSYNTHESIS OF AMYLASES BY  BACILLUS POLYMYXA  CELLS IMMOBILIZED IN CALCIUM ALGINATE AND CHITOSANE.

The aim of the study was to determine optimal conditions for amylases biosynthesis by immobilized Bacillus polymyxa B-20 cells. Composition of the medium and immobilization conditions to a large extent affected the efficiency of enzyme production. Optimal production medium consisted of yeast extract (5-10 g/L), maltose, peptone and mineral salts. Stability of alginate biocatalyst beads was increased by 30 g/L gel concentration, increased bead size and chemical gel modification by glutaraldehyde. Increased quantities of synthesized enzymes were proportional to the increment of initial biomass present in beads, ant to a lesser degree, depended on quantities of immobilized inoculum. In repeated batch culture, the bacteria immobilized in alginate were able to synthesize 32% more enzymes than those immobilized in chitosane. Operational stability of alginate and chitosane beads was maintained for 17 days.

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APPLICATION OF POROUS CERAMIC SINTER AS A SUPPORT FOR IMMOBILIZATION OF SACCHAROMYCES CEREVISIAE YEAST CELLS

ETHANOL FERMENTATION WITH YEAST CELLS IMMOBILIZED ON GRAINS OF POROUS CERAMIC SINTER

IMMOBILISATION OF THE PROTEOLYTIC ENZYME SYSTEM (CHYMOTRYPSIN-EXOPEPTIDASE) IN HIGH-MOLECULAR POLYSACCHARIDES.

CONTINUOUS BIOSYNTHESIS OF AMYLASES BY FREE AND IMMOBILIZED BACILLUS POLYMYXA CELLS

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.