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ORIGINAL ARTICLE
Comparative Characterization of Polysaccharides from Rosa roxburghii Tratt. Fruits Collected from Eight Major Producing Areas of Guizhou: Structure, Physicochemical Properties, and In Vitro Bioactivity
Guangjing Chen 1
,
 
Xuemei Chen 1
,
 
Xianghui Yang 1
,
 
Meiwen Sun 1
,
 
Qingshan Yang 1
 
 
 
More details
Hide details
1
College of Food Science and Engineering, Guiyang University, Guiyang, Guizhou 550005, PR China
 
 
Submission date: 2026-01-18
 
 
Acceptance date: 2026-08-17
 
 
Corresponding author
Guangjing Chen   

College of Food Science and Engineering, Guiyang University, China
 
 
 
KEYWORDS
digestive-enzyme inhibition
homogalacturonan
molecular-weight distribution
pectic polysaccharides
rhamnogalacturonan-I
source-associated variation
 
TOPICS
ABSTRACT
Polysaccharides from Rosa roxburghii Tratt. (RRTP) are predominantly pectic and are considered core bioactive compounds of the fruit. In this study, RRTPs were extracted from fruit of the cultivar ‘Guinong 5’ collected from eight major production areas in Guizhou, China, to evaluate source-associated differences in pectic structural features, physicochemical properties, antioxidant capacity, and inhibitory activity against α-amylase and α-glucosidase. Extraction yields ranged from 3.29% to 4.64%. All RRTPs exhibited typical pectic characteristics, but significant differences were observed in uronic acid content, monosaccharide composition, the distribution of homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) domains, molecular weight, particle behavior, and thermal properties. Among the tested samples, RRTP from Congjiang County (RRTP-CJ) showed the highest galacturonic acid content (49.72 g/100 g) and one of the lowest molecular weights (80.6 kDa). It also exhibited the strongest DPPH•, ABTS•+, and O2•− scavenging activities with half-maximal inhibitory concentration (IC50) values of 0.394, 0.300, and 0.452 mg/mL, respectively. RRTP-CJ also showed the strongest Fe2+-chelating activity, with a half-maximal effective concentration (EC50) of 0.671 mg/mL, and the strongest α-amylase and α-glucosidase inhibition, with IC50 values of 2.425 and 0.747 mg/mL, respectively. Correlation analyses indicated that uronic acid content and HG abundance were positively associated with antioxidant capacity and carbohydrase inhibitory activity, whereas higher molecular weight and extensive RG-I branching were negatively associated with these effects. Principal component analysis further distinguished highly bioactive samples characterized by lower molecular weight and a higher content of galacturonic acid. Overall, substantial source-associated heterogeneity was revealed among RRTPs, and RRTP-CJ was identified as a promising raw material for functional food ingredient development. These findings should be interpreted within the scope of a single cultivar and harvest year, providing a basis for raw-material selection, standardization, and subsequent multi-year validation.
FUNDING
This work was supported by Guizhou Province High Level Innovative Talent Training Program - "1000" talents (No. ZKHT-GCC-[2023]001), Graduate Student Scientific Research Innovation project of Guiyang University (GYU-YJS-[2025]08), and College Students' Innovation and Entrepreneurship Training Program of Guizhou Province (S202010976024).
CONFLICT OF INTEREST
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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