Effects of inuline used as partial fat replacement in cookies containing three different fats were studied. During experiment sensory and instrumental attributes of reference cookies and low fat formulation in which 50% of fat was replaced with inuline were compared. Shortenings used for preparation of cookies differed greatly in saturated fatty acids (SFA) (29.9–57.5%), trans fatty acids (TFA) (0.9–23.1%) and solid fat content (SFC) at 25°C (13.6–31.6%). Replacement of 50% fat with inuline in the formulations enabled obtaining samples with higher instrumental values of texture and resulted in cookies which were harder and less crispy. Also the intensity of sweet taste was sharply reduced by decreasing the fat content of cookie recipes. On the other hand, replacement of 50% of fat with inuline had a little impact on the intensity of buttery taste of the cookies. The lowest intensity of buttery taste was scored in cookies containing shortening with the lowest content of TFA. Concerning the overall sensory quality, the results showed that the replacement of 50% of fat with inuline resulted only in a moderate decrease in the acceptability of the overall sensory quality. The averaged scores of overall sensory quality were generally high and ranged from 7.1 to 9.1. In conclusion, it has been demonstrated that inuline was suitable for inclusion in cookies, as a fat replacer and as a functional ingredient.