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2/2021 vol. 71
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ORIGINAL ARTICLE
Optimization of Distillation Conditions for Improved Recovery of Phthalides from Celery (Apium graveolens L.) Seeds
Adam Kokotkiewicz 1
,
 
Anna Badura 2
,
 
Żaneta Tabaczyńska 2
,
 
Andżelika Lorenc 2
,
 
Adam Buciński 2
,
 
Maria Luczkiewicz 1
 
 
 
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1
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland
 
2
Department of Biopharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Jurasza 2, 85-089 Bydgoszcz, Poland
 
 
Submission date: 2021-02-04
 
 
Final revision date: 2021-05-17
 
 
Acceptance date: 2021-05-18
 
 
Online publication date: 2021-06-07
 
 
Publication date: 2021-06-07
 
 
Corresponding author
Adam Kokotkiewicz   

Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdańsk, Hallera 107, 80-416 Gdańsk, Poland
 
 
Pol. J. Food Nutr. Sci. 2021;71(2):197-210
DOI: https://doi.org/10.31883/pjfns/137612
 
 
Article (PDF)
References (41) Citations (3)
 
KEYWORDS
alkylphthalides
enzyme pretreatment
hydrodistillation
salting-out effect
simultaneous distillation-extraction
size reduction
 
TOPICS
Extraction
Liquid chromatography
Herbs and spices
Essentials oils
Flavours/Aromas
 
ABSTRACT
The essential oil of celery (Apium graveolens) is characterized by exceptionally high content of alkylphthalides. The mentioned compounds exhibit a number of biological effects (including hypotensive, lipid-lowering, neuroprotective, and cytotoxic) and are also responsible for distinctive aroma of the plant. In the current work, parameters of conventional hydrodistillation (HD) and simultaneous distillation-extraction (SDE) were optimized to obtain phthalide-enriched fractions of celery seeds. A positive correlation was shown between hydrodistillation time and improved essential oil and phthalide yields. The 6-h HD of comminuted seeds yielded essential oil (2.9%) with a higher total phthalide content (51%), as compared to the samples collected after 1.5-3.0 h, which gave 2.4-2.7% of oil containing 24.6-39.2% of total phthalides. The oil contained sedanenolide (36.7%), 3-n-butylphthalide (13.1%), and sedanolide (1.1%). A further increase in the total phthalide content was achieved by omitting the size reduction step prior to hydrodistillation (68.8%) and utilization of the salting-out effect (84.3%). Enzyme pretreatment had a negligible effect on essential oil and phthalide yields. The change of distillation mode from HD to SDE significantly increased the oil yield of whole seeds (from 2.8 to 5.8% for 6 h processing) while maintaining its high phthalide content (62.5%), which translated to an increase in the total phthalide yield from 19.4 to 36.0 g/kg.
FUNDING
This research received no external funding.
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