Germination (in light and dark, at 25^oC, for up to 7 days) of cruciferous seeds was studied to determine the optimum conditions to maximize the synthesis of vitamin E in sprouts. In this study, the tocopherol content (a-T, b-T, g-T, d-T) was examined in four species of cruciferous seeds: rapeseed (Brassica napus var. oleifera), white mustard (Sinapis alba L.), radish (Raphanus sativus L.), and small radish (Raphanus sativus var. sativus). Using different light conditions during germination of selected cruciferous seeds, the optimal time to maximize the synthesis of vitamin E was found to be four and five days of germination. Under dark conditions, the optimal germination time was found to be 3 days for small radish and 4 days for radish and rapeseeds. After these times, up to the seventh day of germination, the vitamin E content reached a stable level. A nNegative correlation between a-tocopherol and g-tocopherol content was found for all investigated cruciferous seeds during the germination under light and dark conditions, with only one exception made for rapeseeds. In summary, it was found that a 100-gram portion of individual or mixed fresh cruciferous sprouts is able to cover the Recommended Dietary Allowance for vitamin E in the range of 15.3% - 21.6% for men and 19.1% - 29.5% forwomen.