PROCEDURE FOR THE EVALUATION OF THE OPIOID ACTIVITY OF MILK PRODUCTS

The aim of this work was to prepare the procedure for the evaluation of the opioid activity of milk products. Peptides from acidophilic milk were extracted with methanol/chloroform mixture (2:1 v/v) and from Gouda cheese with the same mixture in a ratio of 1:2 (v/v). The peptide extracts were desalted on Sephacryl S-100 HR column. The opioid activity of the peptide extracts was determined by the measurement of the motoric activity of isolated rabbit intestine (by Magnus method). The identification of the opioid peptides in the peptide extracts was made using thin layer chromatography. β-Casomorphin-5 was identified as opioid peptide in all peptide extracts. For the determination of the β-casomorphin-5 content in the peptide extracts, the standarization of the HPLC method was made. The contents of β-casomorphin-5 in the acidophilic milk and Gouda cheese peptide extracts were: 0.08% and 0.63%, respectively.

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Microbial Profile of Gouda Cheese During Ripening in Two Independent Chambers – a Short Report

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.