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ORIGINAL ARTICLE
Purification and Characterization of a Low Molecular Weight Neutral Non-Starch Polysaccharide from Panax ginseng by Enzymatic Hydrolysis
1
Institute of Grassland Research, Chinese Academy of Agricultural Science, Huhhot, Inner Mongolia, 010010, P.R. China
2
Beijing Key Laboratory of Forest Food Processing and Safety, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, P.R. China
Submission date: 2022-10-19
Acceptance date: 2023-02-01
Online publication date: 2023-02-21
Publication date: 2023-02-21
Corresponding author
Hongxin Wu
Institute of Grassland Research, Chinese Academy of Agricultural Science, Huhhot, Inner Mongolia, 010010, P.R. China
Institute of Grassland Research, Chinese Academy of Agricultural Science, Huhhot, Inner Mongolia, 010010, P.R. China
Pol. J. Food Nutr. Sci. 2023;73(1):70-79
KEYWORDS
TOPICS
ABSTRACT
In this study, a novel neutral non-starch polysaccharide (GP1A) was extracted using hot water from ginseng roots. Chemical characteristics, monosaccharide compositions and structure of GP1A were investigated using scanning electron microscopy, gel permeation chromatography, X-ray diffraction, Fourier transform infrared spectroscopy, gas chromatography with mass spectrometer, one-dimensional and two-dimensional nuclear magnetic resonance. The results indicated that the lyophilized GP1A was a homogeneous polysaccharide with a low molecular weight of 1.03 kDa. The dominating monosaccharides of GP1A were D-glucose, D-galactose, D-mannose, D-xylose and D-arabinose. The structure analysis indicated the main chain residues sequence of GP1A was α-D-Glcp-(6→3)-α-D-Galp-(1→1)-α-D-Glcp-(3→4)-β-D-Manp-(2→2)-β-D-Arap with branch chains of α-D-Glcp substituted at α-D-Galp and β-D-Xylp substituted at β-D-Manp.
FUNDING
This work was financially supported by the Central Public-interest Scientific Institution Basal Research Fund (NO. 1610332022004).
CONFLICT OF INTEREST
The authors have no competing interests.
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CITATIONS (2):
| eISSN: | 2083-6007 |
| ISSN: | 1230-0322 |
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