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3/2025 vol. 75
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ORIGINAL ARTICLE
Quality Differences of Longnan Green Tea Based on Physicochemical Parameters and Volatile Organic Compound Profile
Youqiong Liu 1
,
 
Hongjie Zhang 1
,
 
Junyi Ma 1
,
 
Shengyan Zhang 1
,
 
Xueli Chen 1
,
 
Xuancheng Li 2
 
 
 
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1
College of Life Science, Northwest Normal University, Lanzhou, 730070, China
 
2
Wudu Tea Technology Guidance Station, Longnan, 746000, China
 
 
Submission date: 2025-01-14
 
 
Acceptance date: 2025-08-05
 
 
Online publication date: 2025-08-20
 
 
Publication date: 2025-08-20
 
 
Corresponding author
Junyi Ma   

College of Life Science, Northwest Normal University, Lanzhou, China
 
 
Pol. J. Food Nutr. Sci. 2025;75(3):261-273
DOI: https://doi.org/10.31883/pjfns/209006
 
 
Article (PDF)
Supplementary files
References (56)
 
KEYWORDS
aroma compounds
Camellia sinensis
green tea quality
ion mobility spectrometry
partial least squares-discriminant analysis
principal component analysis
 
TOPICS
Spectrometry
Other techniques
Gas chromatography
Liquid chromatography
Food bioactives
Herbs and spices
Flavours/Aromas
Phenolic compounds
 
ABSTRACT
This study systematically analyzed the chemical composition and profile of volatile organic compounds (VOCs) of green tea samples from three counties (Wenxian, Wudu, and Kangxian) in Longnan, Gansu Province of China, to elucidate their quality differentiation. Principal component analysis (PCA) of 14 physicochemical parameters extracted five principal components accounting for 82.25% of the total variance, indicating that most of the variance within the data set could be explained. Significant differences were found in contents of glutamic acid, tea polysaccharides, water extract, caffeine and free amino acids among the three production areas. Seventy-two VOCs were identified using headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS), and the VOCs were predominantly comprised of aldehydes, ketones, and alcohols. Wenxian green tea contained higher proportions of alcohols and aldehydes, Wudu green tea was rich in terpenes, and Kangxian green tea demonstrated a higher ketone content. Orthogonal partial least squares-discriminant analysis (OPLS-DA) effectively discriminated tea origins with robust model parameters (R²Y=0.937, R²X=0.719, Q²=0.71) and identified 52 key VOCs with variable importance in projection (VIP) >1 and p<0.05 as potential geographical markers for differentiating Longnan green teas from different production areas. These findings establish a quality evaluation framework for Longnan green tea while providing technical support for geographical indication protection and targeted development of characteristic regional tea products.
FUNDING
This work was supported by the Gansu Province Characteristic Advantageous Agricultural Products Evaluation (TYNPZ2022-07), the Key Research and Development Project of Gansu Province (22YF7NA121), and the Central Government Guides Local Science and Technology Development Project of Shandong Province (YDZX2024036).
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest to influence the work reported in this paper.
REFERENCES (56)
1.
Aaqil, M., Peng, C., Kamal, A., Nawaz, T., Zhang, F., Gong, J. (2023). Tea harvesting and processing techniques and its effect on phytochemical profile and final quality of black tea: a review. Foods, 12(24), art. no. 4467. https://doi.org/10.3390/foods1....
CrossRef
 
Google Scholar
 
 
2.
Adnan, M., Ahmad, A., Ahmed, A., Khalid, N., Hayat, I., Ahmed, I. (2013). Chemical composition and sensory evaluation of tea (Camellia sinensis) commercialized in Pakistan. Pakistan Journal of Botany, 45(3), 901-907. https://doi.org/10.29322/PJB.4....
CrossRef
 
Google Scholar
 
 
3.
Aroyeun, S.O. (2013). Crude fibre, water extracts, total ash, caffeine and moisture contents as diagnostic factors in evaluating green tea quality. Italian Journal of Food Science, 25(1), 70-75.
Google Scholar
 
 
4.
Chen, Q.H., Yang, X.B., Hong, P.Z., Liu, M.J., Li, Z.Y., Zhou, C.X., Zhong, S.Y., Liu, S. (2024). GC-MS, GC-IMS, and E-Nose analysis of volatile aroma compounds in wet-marinated fermented golden pomfret prepared using different cooking methods. Foods, 13(3), art. no. 390. https://doi.org/10.3390/foods1....
CrossRef
 
Google Scholar
 
 
5.
Chen, S.N., Kang, J.X., Zhu, H.Q., Wang, K.X., Han, Z.Y., Wang, L.Y., Liu, J.S., Wu, Y., He, P., Yu, Y., Li, B. (2023). L-theanine and immunity: A review. Molecules, 28(9), art. no. 3846. https://doi.org/10.3390/molecu....
CrossRef
 
Google Scholar
 
 
6.
Choi, M.K., Ahn, H.S., Kim, D.E., Lee, D.S., Park, C.S., Kang, C.K. (2025). Effects of varying caffeine dosages and consumption timings on cerebral vascular and cognitive functions: A diagnostic ultrasound study. Applied Sciences, 15(4), art. no. 1703. https://doi.org/10.3390/app150....
CrossRef
 
Google Scholar
 
 
7.
Deka, H., Barman, T., Dutta, J., Devi, A., Tamuly, P., Kumar Paul, R., Karak, T. (2021). Catechin and caffeine content of tea (Camellia sinensis L.) leaf significantly differ with seasonal variation: A study on popular cultivars in North East India. Journal of Food Composition and Analysis, 96, art. no. 103684. https://doi.org/10.1016/j.jfca....
CrossRef
 
Google Scholar
 
 
8.
Deng, S., Zhang, G., Olayemi Aluko, O., Mo, Z., Mao, J., Zhang, H., Liu, X., Ma, M., Wang, Q., Liu, H. (2022). Bitter and astringent substances in green tea: composition, human perception mechanisms, evaluation methods and factors influencing their formation. Food Research International, 157, art. no. 111262. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
9.
Donlao, N., Ogawa, Y. (2019). The influence of processing conditions on catechin, caffeine and chlorophyll contents of green tea (Camelia sinensis) leaves and infusions. LWT – Food Science and Technology, 116, art. no. 108567. https://doi.org/10.1016/j.lwt.....
CrossRef
 
Google Scholar
 
 
10.
GB/T 30987−2020 (2020). Determination of Free Amino Acids in Plants. State Administration for Market Regulation of China, National Standardization Administration Committee of China. http://c.gb688.cn/bzgk/gb/show....
Google Scholar
 
 
11.
GB/T 8314−2013 (2013). Tea − Determination of Free Amino Acids Content. State Administration for Market Regulation of China, National Standardization Administration Committee of China. https://std.sacinfo.org.cn/hom....
Google Scholar
 
 
12.
Ge, Y., Wang, L., Huang, Y., Jia, L., Wang, J. (2024). Characteristic flavor compounds in Guizhou green tea and the environmental factors influencing their formation: Investigation using stable isotopes, electronic nose, and headspace-gas chromatography ion migration spectrometry. LWT – Food Science and Technology, 196, art. no. 115887. https://doi.org/10.1016/j.lwt.....
CrossRef
 
Google Scholar
 
 
13.
Guo, X.Y., Ho, C.T., Schwab, W., Song, C.K., Wan, X.C. (2019). Aroma compositions of large-leaf yellow tea and potential effect of theanine on volatile formation in tea. Food Chemistry, 280, 73-82. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
14.
Huang, D.Z., Li, M.R., Wang, H., Fu, M.Y., Hu, S.D., Wan, X.C., Wang, Z.C., Chen, Q. (2023). Combining gas chromatography-ion mobility spectrometry and olfactory analysis to reveal the effect of filled-N2 anaerobic treatment duration on variation in the volatile profiles of gabaron green tea. LWT – Food Science and Technology, 179, art. no. 114630. https://doi.org/10.1016/j.lwt.....
CrossRef
 
Google Scholar
 
 
15.
ISO 10727:2002 (2002). Tea and Instant Tea in Solid Form − Determination of Caffeine Content − Method Using High-Performance Liquid Chromatography. International Organization for Standardization. https://www.iso.org/standard/3....
Google Scholar
 
 
16.
ISO 14502-2:2005 (2005). Determination of Substances Characteristic of Green and Black Tea − Part 2: Content of Catechins in Green Tea − Method Using High-Performance Liquid Chromatography. International Organization for Standardization. https://www.iso.org/standard/3....
Google Scholar
 
 
17.
ISO 15598:1999 (1999). Tea − Determination of Crude Fibre Content. International Organization for Standardization. https://www.iso.org/standard/2....
Google Scholar
 
 
18.
ISO 19563:2017 (2017). Determination of Theanine in Tea and Instant Tea in Solid Form Using High-Performance Liquid Chromatography. International Organization for Standardization. https://www.iso.org/standard/6....
Google Scholar
 
 
19.
ISO 9768: 1994 (1994). Tea − Determination of Water Extract. International Organization for Standardization. https://www.iso.org/standard/2....
Google Scholar
 
 
20.
Kazan, R.M., Seddik, H.A., Marstani, Z.M., Elsutohy, M.M., Yasri, N.G. (2019). Determination of amino acids content in tea species using liquid chromatography via pre-column fluorescence derivatization. Microchemical Journal, 150, art. no. 104103. https://doi.org/10.1016/j.micr....
CrossRef
 
Google Scholar
 
 
21.
Koch, W., Kukula-Koch, W., Komsta, L., Marzec, Z., Szwerc, W., Glowniak, K. (2018). Green tea quality evaluation based on its catechins and metals composition in combination with chemometric analysis. Molecules, 23(7), art. no. 1689. https://doi.org/10.3390/molecu....
CrossRef
 
Google Scholar
 
 
22.
Kun, J., Yang, Y., Sun, J., Dai, H., Luo, Z., Tong, H. (2025). Characterization of potential aroma compounds in five aroma types of green tea using the sensomics approach. LWT – Food Science and Technology, 215, art. no. 117177. https://doi.org/10.1016/j.lwt.....
CrossRef
 
Google Scholar
 
 
23.
Li, M.M., Sun, M.Q., Ren, W., Man, L.M., Chai, W.Q., Liu, G.Q., Zhu, M.X., Wang, C. (2024). Characterization of volatile compounds in donkey meat by gas chromatography-ion mobility spectrometry (GC-IMS) combined with chemometrics. Food Science of Animal Resources, 44(1), 165-177. https://doi.org/10.5851/kosfa.....
CrossRef
 
Google Scholar
 
 
24.
Liu, N., Shen, S., Huang, L., Deng, G., Wei, Y., Ning, J., Wang, Y. (2023). Revelation of volatile contributions in green teas with different aroma types by GC–MS and GC–IMS. Food Research International, 169, art. no. 112845. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
25.
Pan, S.Y., Nie, Q., Tai, H.C., Song, X.L., Tong, Y.F., Zhang, L.J.F., Wu, X.W., Lin, Z.H., Zhang, Y.Y., Ye, D.Y., Zhang, Y., Wang, X.Y., Zhu, P.L., Chu, Z.S., Yu, Z.L., Liang, C. (2022). Tea and tea drinking: China's outstanding contributions to the mankind. Chinese Medicine, 17(1), art. no. 27. https://doi.org/10.1186/s13020....
CrossRef
 
Google Scholar
 
 
26.
Parvez, S., Wani, I.A. (2024). Exploring the antioxidant realm of green tea: From extraction to fortification. eFood, 5(4), art. no. e172. https://doi.org/10.1002/efd2.1....
CrossRef
 
Google Scholar
 
 
27.
Sari, F., Velioglu, Y.S. (2013). Changes in theanine and caffeine contents of black tea with different rolling methods and processing stages. European Food Research and Technology, 237(2), 229-236. https://doi.org/10.1007/s00217....
CrossRef
 
Google Scholar
 
 
28.
Schaller, T., Schieberle, P. (2020). Quantitation of key aroma compounds in fresh, raw ginger (Zingiber officinale Roscoe) from China and roasted ginger by stable isotope dilution assays and aroma profiling by recombination experiments. Journal of Agricultural and Food Chemistry, 68(51), 15284-15291. https://doi.org/10.1021/acs.ja....
CrossRef
 
Google Scholar
 
 
29.
Shi, S.J., Wang, E.T., Li, C.X., Zhou, H., Cai, M.L., Cao, C.G., Jiang, Y. (2021). Comprehensive evaluation of 17 qualities of 84 types of rice based on principal component analysis. Foods, 10(11), art. no. 2883. https://doi.org/10.3390/foods1....
CrossRef
 
Google Scholar
 
 
30.
Shlens, J. (2014). A tutorial on principal component analysis. ArXiv preprint Arxiv, 1404, art. no. 1100. https://doi.org/10.48550/arXiv....
CrossRef
 
Google Scholar
 
 
31.
Śmiechowska, M., Dmowski, P. (2006). Crude fibre as a parameter in the quality evaluation of tea. Food Chemistry, 94(3), 366-368. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
32.
SN/T 4260−2015 (2015). Determination of Crude Polysaccharides in Plant Source Foods for Export − Phenol-sulfuric Acid Colorimetry. General Administration of Quality Supervision, Inspection and Quarantine of China. http://down.foodmate.net/stand....
Google Scholar
 
 
33.
Tan, J., Engelhardt, U.H., Lin, Z., Kaiser, N., Maiwald, B. (2017). Flavonoids, phenolic acids, alkaloids and theanine in different types of authentic Chinese white tea samples. Journal of Food Composition and Analysis, 57, 8-15. https://doi.org/10.1016/j.jfca....
CrossRef
 
Google Scholar
 
 
34.
Tao, M., Guo, W., Liang, J., Liu, Z. (2025). Unraveling the key cooked off-flavor compounds in thermally sterilized green tea beverages, and masking effect of tea raw material baking. Food Chemistry, 464(Part 1), art. no. 141671. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
35.
Tatsu, S., Matsuo, Y., Nakahara, K., Hofmann, T., Steinhaus, M. (2020). Key odorants in Japanese roasted barley tea (Mugi-Cha) – differences between roasted barley tea prepared from naked barley and roasted barley tea prepared from hulled barley. Journal of Agricultural and Food Chemistry, 68(9), 2728-2737. https://doi.org/10.1021/acs.ja....
CrossRef
 
Google Scholar
 
 
36.
Unno, K., Ikka, T., Yamashita, H., Kameoka, Y., Nakamura, Y. (2025). Stress-relieving effects of Japanese green tea: Evaluation using the molar ratio of caffeine and epigallocatechin gallate to theanine and arginine as an indicator. Foods, 14(1), art. no. 103. https://doi.org/10.3390/foods1....
CrossRef
 
Google Scholar
 
 
37.
Vautz, W., Franzke, J., Zampolli, S., Elmi, I., Liedtke, S. (2018). On the potential of ion mobility spectrometry coupled to GC pre-separation – a tutorial. Analytica Chimica Acta, 1024, 52-64. https://doi.org/10.1016/j.aca.....
CrossRef
 
Google Scholar
 
 
38.
Wang, L., Lin, X, Wang, L.X., Shao, JL., Chen, X.L., Liu, H.C., Mei, W.Q. (2019). Determination and analysis of multifunctional components in tea. Journal of Food Safety and Quality 10(22), 7779-7786. https://doi.org/10.19812/j.cnk....
CrossRef
 
Google Scholar
 
 
39.
Wang, Q., Yang, X., Zhu, C., Liu, G., Sun, Y., Qian, L. (2022). Advances in the utilization of tea polysaccharides: Preparation, physicochemical properties, and health benefits. Polymers, 14(14), art. no. 2775. https://doi.org/10.3390/polym1....
CrossRef
 
Google Scholar
 
 
40.
Wang, S.Q., Chen, H.T., Sun, B.G. (2020). Recent progress in food flavor analysis using gas chromatography-ion mobility spectrometry (GC-IMS). Food Chemistry, 315, art. no. 126158. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
41.
Wei, K., Wang, L., Zhou, J., He, W., Zeng, J., Jiang, Y., Cheng, H. (2011). Catechin contents in tea (Camellia sinensis) as affected by cultivar and environment and their relation to chlorophyll contents. Food Chemistry, 125(1), 44-48. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
42.
Wu, C.Y., Zhang, H., Zhang, H.M., Sun, J.B., Song, Z. (2024). Detection of Ganoderma lucidum spore oil adulteration using chemometrics based on a flavor fingerprint by HS-GC-IMS. European Food Research and Technology, 250(6), 1683-1693. https://doi.org/10.1007/s00217....
CrossRef
 
Google Scholar
 
 
43.
Xiao, K., Shi, Y., Liu, S., Chen, Y., Ni, D., Yu, Z. (2023). Compositions and antioxidant activity of tea polysaccharides extracted from different tea (Camellia sinensis L.) varieties. Foods, 12(19), art. no. 3584. https://doi.org/10.3390/foods1....
CrossRef
 
Google Scholar
 
 
44.
Xiao, Y., Huang, Y.X., Chen, Y.L., Xiao, L.K., Zhang, X.L., Yang, C.H.W., Li, Z.J., Zhu, M., Liu, Z., Wang, Y. (2022). Discrimination and characterization of the volatile profiles of five Fu brick teas from different manufacturing regions by using HS-SPME/GC-MS and HS-GC-IMS. Current Research in Food Science, 5, 1788-1807. https://doi.org/10.1016/j.crfs....
CrossRef
 
Google Scholar
 
 
45.
Xu, C., Liang, L., Li, Y., Yang, T., Fan, Y., Mao, X., Wang, Y. (2021). Studies of quality development and major chemical composition of green tea processed from tea with different shoot maturity. LWT – Food Science and Technology, 142, art. no. 111055. https://doi.org/10.1016/j.lwt.....
CrossRef
 
Google Scholar
 
 
46.
Ye, Y.L., Yan, J.N., Cui, J.L., Mao, S.H., Li, M.F., Liao, X.L., Tong, H.R. (2018). Dynamic changes in amino acids, catechins, caffeine and gallic acid in green tea during withering. Journal of Food Composition and Analysis, 66, 98-108. https://doi.org/10.1016/j.jfca....
CrossRef
 
Google Scholar
 
 
47.
Yin, P., Kong, Y.-S., Liu, P.P., Wang, J.J., Zhu, Y., Wang, G.M., Sun, M.-F., Chen, Y., Guo, G.Y., Liu, Z.H. (2022). A critical review of key odorants in green tea: Identification and biochemical formation pathway. Trends in Food Science & Technology, 129, 221-232. https://doi.org/10.1016/j.tifs....
CrossRef
 
Google Scholar
 
 
48.
Yu, P., Yeo, A.S.L., Low, M.Y., Zhou, W. (2014). Identifying key non-volatile compounds in ready-to-drink green tea and their impact on taste profile. Food Chemistry, 155, 9-16. https://doi.org/10.1016/j.food....
CrossRef
 
Google Scholar
 
 
49.
Yu, X.-L., Sun, D.-W., He, Y. (2020). Emerging techniques for determining the quality and safety of tea products: A review. Comprehensive Reviews in Food Science and Food Safety, 19(5), 2613–2638. https://doi.org/10.1111/1541-4....
CrossRef
 
Google Scholar
 
 
50.
Zeng, L.T., Watanabe, N., Yang, Z.Y. (2019). Understanding the biosyntheses and stress response mechanisms of aroma compounds in tea (Camellia sinensis) to safely and effectively improve tea aroma. Critical Reviews in Food Science and Nutrition, 59(14), 2321-2334. https://doi.org/10.1080/104083....
CrossRef
 
Google Scholar
 
 
51.
Zhang, L., Cao, Q.Q., Granato, D., Xu, Y.Q., Ho, C.T. (2020). Association between chemistry and taste of tea: A review. Trends in Food Science & Technology, 101, 139-149. https://doi.org/10.1016/j.tifs....
CrossRef
 
Google Scholar
 
 
52.
Zheng, K.W., Luo, X.Q., Song, S.J., Dong, R.J., Wang, P.M., Xu, B.B., Xu, J.Z. (2024). Qualitative and quantitative analysis for monitoring the fishy odor of anchovy oil. International Journal of Food Engineering, 20(4), 279-289. https://doi.org/10.1515/ijfe-2....
CrossRef
 
Google Scholar
 
 
53.
Zheng, Z.X., Zhang, Y.P., Li, Y.X., Yang, X.Q., Du, J.R. (2021). Quality evaluation of Luzhou high-mountain green tea extract and optimization of its extraction process. Contemporary Chemical Industry, 50(4), 848-853+872.
Google Scholar
 
 
54.
Zhou, Q.Y., Liang, D.X., Ling, C.J., Gao, L.Y., Ling, Z. (2024). Characterization of the volatile components in the processing of Citri Exocarpium Rubrum black tea based on HS-SPME and GC/MS. Food Science & Nutrition, 12(10), 7913-7923. https://doi.org/10.1002/fsn3.4....
CrossRef
 
Google Scholar
 
 
55.
Zhu, B.Y., Zhang, J., Li, J.Y., Fang, S.M., Zhang, Z.Z., Wang, R.J., Deng, W.W. (2021). Aroma profile of Jinmudan tea produced using Camellia sinensis, cultivar Jinmudan using solid-phase microextraction, gas chromatography-mass spectrometry, and chemometrics. European Food Research and Technology, 247(5), 1061-1082. https://doi.org/10.1007/s00217....
CrossRef
 
Google Scholar
 
 
56.
Zhu, L.J., Ou, F.L., Xiang, Y., Wang, B., Mao, Y.C., Zhu, L.F., Zhang, Q., Lei, C. (2024). Detection and comparison of volatile organic compounds in four varieties of hawthorn using HS-GC-IMS. Separations, 11(4), art. no. 100. https://doi.org/10.3390/separa....
CrossRef
 
Google Scholar
 
 
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