ANTIOXIDANT ACTIVITY OF PHENOLIC FRACTIONS OF LITSEA MONOPETALA (PERSIMON-LEAVED LITSEA) BARK EXTRACT.
 
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Pol. J. Food Nutr. Sci. 2008;58(2):229–233
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ABSTRACT
Phenolic compounds were extracted from Litsea monopetala bark using methanol. Four fractions (I-IV) were separated from extract on a Sephadex LH-20 column using methanol as the mobile phase evaluated for their total antioxidant activity, antiradical activity against DPPH·, (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents of the fractions were determined. The highest relative content in the crude extract was found for fraction I. The content of total phenolics in fractions ranged from 169 mg/g (fraction I) to 753 mg/g (fraction II). Condensed tannins were detected in fractions II-IV. The fractions exhibited the total antioxidant activity from 1.90 mmol Trolox/g (fraction I) to 7.06 mmol Trolox/g (fraction IV). Fraction III was characterised by the highest antiradical activity against DPPH (EC50 = 0.011 mg/assay). Fractions II and III showed the strongest reducing power which was app. four times higher than that of fractions I. The presence of three main phenolic compounds in fraction I was confirmed using RP-HPLC.
ISSN:1230-0322