APPLICATION OF HPLC-DAD FOR DETECTION OF PHENOLIC COMPOUNDS BOUND TO RAPESEED 12S GLOBULIN - A SHORT REPORT
 
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Pol. J. Food Nutr. Sci. 2004;54(3):233–236
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ABSTRACT
Cruciferin (12S globulin) was extracted and purified from rapeseed and analysed using the HPLC method with a diode array UV detector. The reversed phase column and a gradient of acetonitrile in water were applied for the separation of protein and phenolic constituents. The chromatogram was characterised with a broad peak of globulin with retention time of 24.65 min. Additional peaks showed that phenolic compounds can occur as a complex with rapeseed 12S globulin. Two compounds were identified as sinapic acid derivatives. Their DAD-UV spectra possessed maxima at 328 and 336 nm.
ISSN:1230-0322