Comparison of the Biological Activity of Crude Polysaccharide Fractions Obtained from Cedrela sinensis Using Different Extraction Methods
 
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Pol. J. Food Nutr. Sci. 2018;68(4):327–334
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ABSTRACT:
Four preparations of polysaccharide were obtained from Cedrela sinensis using four methods of extraction, and finally precipitation with ethanol. Hot-water and processes assisted by ultrasound and enzymes (Shearzyme or Viscozyme) were carried out. The yield of polysaccharide fraction extracted with the enzyme-assisted method using Shearzyme (P-EAS) was the highest at 10.53%. All crude saccharides were composed of six monosaccharides including arabinose, galactose, glucose, mannose, rhamnose, and xylose. Crude polysaccharide fraction extracted by hot water showed the highest DPPH• and ABTS•+ scavenging activities as well as α-amylase inhibitory activity. All fractions showed higher retardation effects on glucose and bile acid absorption using the in vitro dialysis system compared to the control (without polysaccharide fraction); specifically, the glucose retardation index of P-EAS was similar to that of carboxymethylcellulose. Overall, crude polysaccharide fractions obtained from C. sinensis can be used as functional ingredients owing to their excellent functional properties.