Search for Author, Title, Keyword
ORIGINAL ARTICLE
Centella asiatica (L.) Urb. Stem-Enriched Cookies: Reducing Glycemic Index and Improving Phenolic and Chlorophyll Release in the Intestine
,
 
,
 
,
 
,
 
 
 
More details
Hide details
1
Ho Chi Minh City University of Technology, Ho Chi Minh City University of Technology (HCMUT), 268 Ly Thuong Kiet Street, Dien Hong Ward, Ho Chi Minh City, Vietnam
 
2
Vietnam National University – Ho Chi Minh City (VNU-HCM), Vo Truong Toan Street, Linh Xuan Ward, Ho Chi Minh City, Vietnam
 
 
Submission date: 2026-04-22
 
 
Acceptance date: 2026-10-01
 
 
Corresponding author
Van Viet Man Le   

Department of Food Technology, Ho Chi Minh City University of Technology (HCMUT) Vietnam National University Ho Chi Minh City (VNU-HCM), 268 Ly Thuong Kiet Street, District 10, 70000, Ho Chi Minh City, Viet Nam
 
 
 
KEYWORDS
TOPICS
ABSTRACT
Asiatic pennywort (Centella asiatica (L.) Urb.) stems, although rich in dietary fiber and phenolic compounds, are usually discarded during processing. This study investigated the potential of Asiatic pennywort stem powder (APSP) as a partial substitute for soft wheat flour in cookie production at varying levels (0–20%, w/w). Nutritional composition, physicochemical properties, glycemic response, release of phenolics and chlorophylls at the intestinal stage, and overall acceptability of the fortified cookies were analyzed and compared. The use of APSP markedly increased dietary fiber, total phenolic, total flavonoid, and chlorophyll contents of the fortified cookies. Consumer evaluation confirmed acceptance of cookies produced by substituting soft wheat flour with APSP up to 15% (w/w). The APSP fortification effectively reduced the glycemic index of cookies, placing them within the low-GI category of food products, and significantly improved the release of total phenolics (5.8–7.7 fold) and total flavonoids (4.7–6.5 fold) at the intestinal phase when compared to the control sample. Overall, the findings demonstrate that APSP is a promising sustainable ingredient for the development of health-oriented baked products, while supporting environmental sustainability and a circular economy.
ACKNOWLEDGEMENTS
We acknowledge Ho Chi Minh City University of Technology (HCMUT), VNU-HCM for supporting this study.
FUNDING
This study did not receive any external funding.
CONFLICT OF INTEREST
All authors declare that we have no conflicts of interest.
ADDITIONAL INFORMATION
The study protocol was approved the Ethics Review Board at the University of Social Sciences and Humanities, Vietnam National University – Ho Chi Minh City (VNU-HCM); approval number 01/GCN-XHNV-HDDDNC from November 21st, 2022.
REFERENCES (39)
1.
Alimentarius, C. (1997). Codex Alimentarius. Guidelines for use of nutrition and health claims (CAC/GL 23-1997).
 
2.
Alqahtani, N.K., Alnemr, T.M., Almadhi, F.H., Aboufarrag, H.T. (2024). Development and evaluation of calorie-reduced functional cookies enriched with date press cake. Journal of Food Processing and Preservation, 2024(1), art. no. 3648845. https://doi.org/10.1155/2024/3....
 
3.
AOAC (2000). Official Methods of Analysis (18th ed.). The Association of Official Analytical Chemists International, Rockville, MD, USA.
 
4.
Atkinson, F.S., Brand-Miller, J.C., Foster-Powell, K., Buyken, A.E., Goletzke, J. (2021). International tables of glycemic index and glycemic load values 2021: a systematic review. The American Journal of Clinical Nutrition, 114(5), 1625-1632. https://doi.org/10.1093/ajcn/n....
 
5.
Ayele, D.T., Akele, M.L., Melese, A.T. (2022). Analysis of total phenolic contents, flavonoids, antioxidant and antibacterial activities of Croton macrostachyus root extracts. BMC Chemistry, 16(1), art. no. 30. https://doi.org/10.1186/s13065....
 
6.
Benzie, I.F.F., Devaki, M. (2018). Chapter 5: The ferric reducing/antioxidant power (FRAP) assay for non-enzymatic antioxidant capacity: concepts, procedures, limitations and applications. In R. Apak, E. Capanoglu, F. Shahidi (Eds). Measurement of Antioxidant Activity & Capacity, John Wiley & Sons, pp. 77-106. https://doi.org/10.1002/978111....
 
7.
Boulos, N.N., Greenfield, H., Wills, R.B.H. (2000). Water holding capacity of selected soluble and insoluble dietary fibre. International Journal of Food Properties, 3(2), 217-231. https://doi.org/10.1080/109429....
 
8.
Brodkorb, A., Egger, L., Alminger, M., Alvito, P., Assunção, R., Ballance, S., Bohn, T., Bourlieu-Lacanal, C., Boutrou, R., Carrière, F., Clemente, A., Corredig, M., Dupont, D., Dufour, C., Edwards, C., Golding, M., Karakaya, S., Kirkhus, B., Le Feunteun, S., Lesmes, U., Macierzanka, A., Mackie, A. R., Martins, C., Marze, S., McClements, D.J., Ménard, O., Minekus, M., Portmann, R., Santos, C.N., Souchon, I., Singh, R.P., Vegarud, G.E., Wickham, M.S.J., Weitschies, W., Recio, I. (2019). INFOGEST static in vitro simulation of gastrointestinal food digestion. Nature Protocols, 14(4), 991-1014. https://doi.org/10.1038/s41596....
 
9.
Chi, C., Li, X., Zhang, Y., Chen, L., Li, L., Wang, Z. (2017). Digestibility and supramolecular structural changes of maize starch by non-covalent interactions with gallic acid. Food & Function, 8(2), 720-730. https://doi.org/10.1039/C6FO01....
 
10.
Das, A.J. (2011). Review on nutritional, medicinal and pharmacological properties of Centella asiatica (Indian pennywort). Journal of Biologically Active Products from Nature, 1(4), 216-228. https://doi.org/10.1080/223118....
 
11.
Grzelczyk, J., Drożdżyński, P., Budryn, G., Czarnecki, A., Paprocka, Z., Gałązka-Czarnecka, I. (2025). High-fiber cookies with bamboo flour and edible flowers: Evaluation of structural properties, phenolic content, antioxidant activity and nutritional value. LWT – Food Science and Technology, 216, art. no. 117321. https://doi.org/10.1016/j.lwt.....
 
12.
Gulcin, İ., Alwasel, S.H. (2023). DPPH radical scavenging assay. Processes, 11(8), art. no. 2248. https://doi.org/10.3390/pr1108....
 
13.
Hariharan, R., Odjidja, E. N., Scott, D., Shivappa, N., Hébert, J.R., Hodge, A., de Courten, B. (2022). The dietary inflammatory index, obesity, type 2 diabetes, and cardiovascular risk factors and diseases. Obesity Reviews, 23(1), art. no. e13349. https://doi.org/10.1111/obr.13....
 
14.
He, Y., Wang, B., Wen, L., Wang, F., Yu, H., Chen, D., Su, X., Zhang, C. (2022). Effects of dietary fiber on human health. Food Science and Human Wellness, 11(1), 1-10. https://doi.org/10.1016/j.fshw....
 
15.
Ibrahim, M.S., Ahmad, A., Nadeem, M., Ranjha, M.M.A.N., Nadeem, M.A., Malik, F., Teferra, T.F. (2023). Farinograph, mixograph, and pasting properties of oat fiber-incorporated dough and sensory attributes of fiber-incorporated biscuits. Cogent Food & Agriculture, 9(1), art. no. 2244811. https://doi.org/10.1080/233119....
 
16.
Jose, M., Himashree, P., Sengar, A.S., Sunil, C.K. (2022). Valorization of food industry by-product (pineapple pomace): A study to evaluate its effect on physicochemical and textural properties of developed cookies. Measurement: Food, 6, art. no. 100031. https://doi.org/10.1016/j.meaf....
 
17.
Kaur, J., Kaur, K., Singh, B., Singh, A., Sharma, S. (2022). Insights into the latest advances in low glycemic foods, their mechanism of action and health benefits. Journal of Food Measurement and Characterization, 16(1), 533-546. https://doi.org/10.1007/s11694....
 
18.
Long, D.Q., Trinh, M.T.T., Nguyen, V.H., Tran, T.T.T., Ton, N.M.N., Le, V.V.M. (2025). Use of transglutaminase at different concentrations and incubation times for quality improvement of high-fiber cracker fortified with soybean hulls. Applied Food Research, 5(2), art. no. 101298. https://doi.org/10.1016/j.afre....
 
19.
Lucas-González, R., Ángel Pérez-Álvarez, J., Moscaritolo, S., Fernández-López, J., Sacchetti, G., Viuda-Martos, M. (2021). Evaluation of polyphenol bioaccessibility and kinetic of starch digestion of spaghetti with persimmon (Dyospyros kaki) flours coproducts during in vitro gastrointestinal digestion. Food Chemistry, 338, art. no. 128142. https://doi.org/10.1016/j.food....
 
20.
Mai, T.H.A., Tran, T.T.T., Le, V.V.M. (2022). Use of pitaya peel powder for partial replacement of wheat flour in cookie making: Effects of particle size of pitaya peel powder on the product quality. Journal of Food Processing and Preservation, 46(1), art. no. e16214. https://doi.org/10.1111/jfpp.1....
 
21.
Manye, S.J., Saleh, J.S., Ishaya, H.B., Chiroma, S.M., Attah, M.O.O., Dibal, N.I. (2023). Phytochemical screening and in-vitro antioxidant activities of aqueous and methanol extracts of Aloe vera. Pharmacological Research - Modern Chinese Medicine, 8, art. no. 100291. https://doi.org/10.1016/j.prmc....
 
22.
Mudgil, D., Barak, S., Khatkar, B.S. (2017). Cookie texture, spread ratio and sensory acceptability of cookies as a function of soluble dietary fiber, baking time and different water levels. LWT – Food Science and Technology, 80, 537-542. https://doi.org/10.1016/j.lwt.....
 
23.
Naknaen, P., Itthisoponkul, T., Sondee, A., Angsombat, N. (2016). Utilization of watermelon rind waste as a potential source of dietary fiber to improve health promoting properties and reduce glycemic index for cookie making. Food Science and Biotechnology, 25(2), 415-424. https://doi.org/10.1007/s10068....
 
24.
Nammakuna, N., Barringer, S.A., Ratanatriwong, P. (2016). The effects of protein isolates and hydrocolloids complexes on dough rheology, physicochemical properties and qualities of gluten-free crackers. Food Science & Nutrition, 4(2), 143-155. https://doi.org/10.1002/fsn3.2....
 
25.
Nguyen, N.D.T., Phan, T.T.H., Tran, T.T.T., Ton, N.M.N., Vo, D.L.T., Man Le, V.V. (2022). Enzymatic treatment of spent green tea leaves and their use in high-fibre cookie production. Food Technology and Biotechnology, 60(3), 396-405. https://doi.org/10.17113/ftb.6....
 
26.
Nickel, D.V., Jannasch, F., Inan-Eroglu, E., Kuxhaus, O., Schulze, M.B. (2024). Healthy food diversity and the risk of major chronic diseases in the EPIC-Potsdam study. Scientific Reports, 14(1), art. no. 28635. https://doi.org/10.1038/s41598....
 
27.
Ozgoren, E., Isik, F., Yapar, A. (2019). Effect of Jerusalem artichoke (Helianthus tuberosus L.) supplementation on chemical and nutritional properties of crackers. Journal of Food Measurement and Characterization, 13(4), 2812-2821. https://doi.org/10.1007/s11694....
 
28.
Pareek, S., Sagar, N.A., Sharma, S., Kumar, V., Agarwal, T., González-Aguilar, G.A., Yahia, E.M. (2017). Chapter 14: Chlorophylls: Chemistry and biological functions. In E.M. Yahia (Ed.), Fruit and Vegetable Phytochemicals: Chemistry and Human Health (2nd edition).. Wiley-Blackwell (John Wiley & Sons Ltd.), pp. 269-284. https://doi.org/10.1002/978111....
 
29.
Pinto, D., Moreira, M.M., Švarc-Gajić, J., Vallverdú-Queralt, A., Brezo-Borjan, T., Delerue-Matos, C., Rodrigues, F. (2023). In-vitro gastrointestinal digestion of functional cookies enriched with chestnut shells extract: Effects on phenolic composition, bioaccessibility, bioactivity, and α-amylase inhibition. Food Bioscience, 53, art. no. 102766. https://doi.org/10.1016/j.fbio....
 
30.
Roshanak, S., Rahimmalek, M., Goli, S. A. H. (2016). Evaluation of seven different drying treatments in respect to total flavonoid, phenolic, vitamin C content, chlorophyll, antioxidant activity and color of green tea (Camellia sinensis or C. assamica) leaves. Journal of Food Science and Technology, 53(1), 721-729. https://doi.org/10.1007/s13197....
 
31.
Sun, L., Miao, M. (2020). Dietary polyphenols modulate starch digestion and glycaemic level: a review. Critical Reviews in Food Science and Nutrition, 60(4), 541-555. https://doi.org/10.1080/104083....
 
32.
Sun, X., Scanlon, M.G., Nickerson, M.T., Koksel, F. (2023). The entrainment and evolution of gas bubbles in bread dough – A review. Cereal Chemistry, 100(5), 1031-1047. https://doi.org/10.1002/cche.1....
 
33.
Tran, T.T.T., Ngo, L.H.N., Ton, N.M.N., Le, T.H.N., Le, T.T., Le, V.V.M. (2024). High-fiber crackers supplemented with asparagus hard-stem: impacts of supplementation ratios and water amounts in cracker recipe on the product quality. Polish Journal of Food and Nutrition Sciences, 74(2), 162-168. https://doi.org/10.31883/pjfns....
 
34.
Trinidad, T.P., Mallillin, A.C., Sagum, R.S., Encabo, R.R. (2010). Glycemic index of commonly consumed carbohydrate foods in the Philippines. Journal of Functional Foods, 2(4), 271-274. https://doi.org/10.1016/j.jff.....
 
35.
Udeh, C.C., Malomo, S.A., Ijarotimi, O.S. (2024). In vitro antioxidants and anti-inflammatory potentials of high protein-fibre cookies produced from whole wheat, sweet potato, rice bran and peanut composite flour blends. Food Production, Processing and Nutrition, 6(1), art. no. 73. https://doi.org/10.1186/s43014....
 
36.
Viera, I., Herrera, M., Roca, M. (2022). Influence of food composition on chlorophyll bioaccessibility. Food Chemistry, 386, art. no. 132805. https://doi.org/10.1016/j.food....
 
37.
Weldeyohanis Gebremariam, F., Tadesse Melaku, E., Sundramurthy, V.P., Woldemichael Woldemariam, H. (2024). Development of functional cookies form wheat-pumpkin seed based composite flour. Heliyon, 10(2), art. no. e24443. https://doi.org/10.1016/j.heli....
 
38.
Yoo, J.Y., Kim, Y. (2009). Survey of cookie consumption and nutrition labelling of cookie consumed in high school students. Korean Journal of Community Nutrition, 14(2), 147-157 (in Korean, English abstract).
 
39.
Zhu, F. (2015). Interactions between starch and phenolic compound. Trends in Food Science & Technology, 43(2), 129-143. https://doi.org/10.1016/j.tifs....
 
eISSN:2083-6007
ISSN:1230-0322
Journals System - logo
Scroll to top