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Effects of Four-Week Intake of Blackthorn Flower Extract on Mice Tissue Antioxidant Status and Phenolic Content
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Biology Department, Faculty of Science, University of Zagreb, Croatia
Department of Animal Physiology, Faculty of Science Univeristy of Zagreb, Croatia
Department of Food Quality Control Laboratory for Food Chemistry and Biochemistry, Faculty of Food Technology and Biotechnology University of Zagreb, Croatia
Department of food toxicology, Faculty of Food Technology and Biotechnology University of Zagreb, Croatia
Department of Food Engineering Laboratory for Technology of Fruits and Vegetables Preservation and Processing, Faculty of Food Technology and Biotechnology University of Zagreb, Croatia
Submission date: 2020-04-30
Final revision date: 2020-09-29
Acceptance date: 2020-10-05
Online publication date: 2020-12-09
Publication date: 2020-12-09
Corresponding author
Domagoj Đikić   

Department of Animal Physiology, Faculty of Science Univeristy of Zagreb, Rooseveltov trg 6, 10000, Zagreb, Croatia
Pol. J. Food Nutr. Sci. 2020;70(4):361-375
The study examined the antioxidative physiological effects of phenolics from an ethanol-water extract of blackthorn flowers orally administrated to C57/BL6 mice for 28 days in daily doses of 25 mg of total phenolics/kg body weight. Contents of phenolics in the intestine, liver, and kidneys collected after 1, 7, 14, 21, and 28 days of extract administration were analyzed by UPLC-MS/MS method. In the same tissues, the antioxidative properties were determined as ferric reducing antioxidant power (FRAP), ABTS•+ scavenging activity, content of reduced glutathione (GSH), and activity of superoxide dismutase (SOD) and catalase (CAT). The lipid peroxidation in tissues was also evaluated by thiobarbituric acid reactive substances (TBARS) assay. The exposed mice (compared to the control ones) had a lower content of TBARS in all tissues mostly on the third/fourth week of daily consumption. SOD activity and GSH content increased on the 28th day in tissues. CAT activity was higher only in the liver after one week of consumption but remained unchanged in other organs throughout the experiment. Phenolic profiles were different in individual tissues. The most prominent increases compared to the control were determined for contents of 3-O-feruloylquinic acid, 4-O-p-coumaroylqiunic acid, kaempferol pentoside, and quercetin rhamnoside in the intestine; for ferulic acid and quercetin 3-O-rutinoside in the liver; and for quercetin 3-O-rutinoside, ferulic acid, and 4-O-p-coumaroylquinic acid in the kidneys. The screened phenolics with different distribution in tissues could be responsible for slight differences in the recorded antioxidative effects.
We are grateful to the staff of the Animal Breeding Facility of the Department of Animal Physiology Faculty of Science, University of Zagreb, for their help during conduction of the in vivo experiment and to Dr. Zoran Zoric and Dr. Sandra Pedisic for their great effort during the preparation of UPLC-MS/MS data and results.
This work was supported by the project “Bioactive molecules of medical plants as natural antioxidants, microbicides, and preservatives” (KK., co-financed by the Croatian Government and the European Union through the European Regional Development Fund-Operational Programme Competitiveness and Cohesion (KK.
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