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ACTIVITY AND IMMUNOREACTIVITY OF LIPASE IN STORED SUNFLOWER SEEDS
 
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Publication date: 2003-03-31
 
 
Pol. J. Food Nutr. Sci. 2003;53(Special issue 1s):57-59
 
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ABSTRACT
During the storage of sunflower seeds with moisture content adjusted to 6 and 18%, activity (by the diffusion method) and immunoreactivity (by the immunoblotting method) of lipase were examined. The activity of sunflower lipase ranged from 28,4 to 37,5 A.U./mg protein and from 24,5 to 31,4 A.U./mg protein during storage of sunflower seeds with moisture content adjusted to 6% and 18%, respectively. Protein fraction characterized by molecular weights of 78 kD on electrophoregrams of protein extract from sunflower seeds with moisture content 6% cross reacted with the anti-lipase antibodies. The presence of immunoreactive proteins however was not detected in the extracts from sunflower seeds with 18% moisture content. The enzyme immunoreactive properties appear to be affected by moisture content to a higher extent as compared to its activity, so no relationship between the enzyme activity and immunoreactivity is observed.
eISSN:2083-6007
ISSN:1230-0322
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