Phenolic compounds were extracted from flaxseed using 80% methanol. Then the crude extract was hydrolysed at basic condition. SDG was separated from so prepared extract using a semi-preparative HPLC. Chemical structure of SDG was confirmed by ESI-MS. Micellar electrokinetic chromatography (MEKC) was used for separation of phenolics from the extract after hydrolysis. MEKC was found to offer a quicker analysis with high separation efficiency than the conventional HPLC method. Retention time for SDG was noted at 12.25 min (sample) and 11.95 (standard).
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