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Macro- and Micro-Nutrient Composition and Antioxidant Activity of Chickpea and Pea Accessions
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Department of Soil, Plant and Food Science (DISSPA), University of Bari Aldo Moro, via Amendola, 165/A, I-70126 Bari, Italy
Department of Bioscience, Biotechnologies and Biopharmaceutics (DBBB) University of Bari Aldo Moro, via Amendola, 165/A, I-70126 Bari, Italy
Faculty of Commodity Science, Poznań University of Economics and Business, al. Niepodległości 10, 61-875 Poznań, Poland
Research Centre for Animal Production and Aquaculture, Council for Agricultural Research and Economics (CREA), viale Piacenza, 29, I-26900 Lodi, Italy
Carmine Summo   

Department of the Soil, Plant and Food Science, Univerisity of Bari Aldo Moro, Via G. Amendola 165/A, 70037, Bari, Italy
Submission date: 2020-12-07
Final revision date: 2021-04-05
Acceptance date: 2021-04-15
Epidemiological studies reported an inverse association between the consumption of legumes and the incidence of age-related diseases. This trend could be attributed to the presence of antioxidant compounds, especially phenolic and flavonoid compounds. In this paper, five pea (Pisum sativum L.) and twelve chickpea (Cicer arietinum L.) accessions, having different characteristics and geographical origin, were characterised in terms of antioxidant activity, as well as macro- and micro-nutrient composition. The antioxidant activity has been evaluated using both DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging capacity assays. Chickpea and pea accessions showed a different behaviour in the presence of these different radicals. Chickpea accessions were characterised by significantly higher DPPH• scavenging activity, while peas showed a significantly higher value of antioxidant activity evaluated using the ABTS assay. Pea accessions had the highest content of total phenolic compounds, Zn, and Cu. A positive correlation was found between some minerals, such as Zn, Cu and P, and the ABTS•+ scavenging activity. Black and brown chickpea accessions showed significantly higher contents of anthocyanins, Mn, Mg, and Ca, which were positively correlated with the antioxidant activity assessed with the DPPH assay. Despite the dataset investigated in our study included a limited number of accessions, it was possible to highlight the influence of the chemical composition on the antioxidant activity due to the high phenotypic diversity found between the accessions, emphasising the importance of selecting the antioxidant activity assay according to the matrix to be evaluated.
This research has been performed within the project “LEgume GEnetic REsources as a tool for the development of innovative and sustainable food TEchnological system” supported under the “Thought for Food” Initiative by Agropolis Fondation (through the “Investissements d’avenir” programme with reference number ANR-10-LABX-0001-01), Fondazione Cariplo, and Daniel & Nina Carasso Foundation.
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