APPLICATION OF HPLC-DAD FOR DETECTION OF PHENOLIC COMPOUNDS BOUND TO RAPESEED 12S GLOBULIN - A SHORT REPORT

Cruciferin (12S globulin) was extracted and purified from rapeseed and analysed using the HPLC method with a diode array UV detector. The reversed phase column and a gradient of acetonitrile in water were applied for the separation of protein and phenolic constituents. The chromatogram was characterised with a broad peak of globulin with retention time of 24.65 min. Additional peaks showed that phenolic compounds can occur as a complex with rapeseed 12S globulin. Two compounds were identified as sinapic acid derivatives. Their DAD-UV spectra possessed maxima at 328 and 336 nm.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.