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ORIGINAL ARTICLE
Influence of Cellulase Pretreatment on the Quality Characteristics, Total Phenolic Content, and Antioxidant Capacity of Hydraulic Press-Extracted Grapeseed Oil
Katheryn S. Lezama Guerra 1
,
 
Gloria J. Pascual Chagman 1,2
,
 
Andrea Brandolini 3
,
 
Alyssa Hidalgo 4
,
 
Marco A. Florez-Jalixto 1
,
 
Christian R. Encina-Zelada 1,2
 
 
 
More details
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1
Departamento de Tecnología de Alimentos y Productos Agropecuarios , Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima 15024, Peru
 
2
Instituto de Investigación de Bioquímica y Biología Molecular, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina , Lima 15024, Peru
 
3
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria - Unità di Ricerca per la Zootecnia e l’Acquacoltura (CREA-ZA), viale Piacenza 29, 26900 Lodi, Italy
 
4
Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via Celoria 2, 20133 Milan, Italy
 
 
Submission date: 2025-07-14
 
 
Acceptance date: 2025-11-12
 
 
Corresponding author
Katheryn S. Lezama Guerra   

Departamento de Tecnología de Alimentos y Productos Agropecuarios , Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima 15024, Peru
 
 
 
KEYWORDS
antiradical activity
enzymatic treatment
physicochemical characteristics
Vitis labrusca × Vitis vinifera
 
TOPICS
ABSTRACT
Grape seeds, a by-product of winemaking, are a valuable source of oil, rich in essential fatty acids and bioactive compounds. This study evaluated the influence of cellulase pretreatment on the hydraulic press extraction of oil from Vitis labrusca × Vitis vinifera grape seeds. Grape seed powder (GSP) was treated with cellulase at enzyme-to-substrate ratios of 0.5 and 2 g/100 g for 4 h and 18 h. The extracted oils were analysed for yield, fatty acid composition, total phenolic content, antioxidant capacity, and physicochemical properties. Enzymatic pretreatment significantly enhanced oil recovery compared to the untreated control, with extraction efficiency increasing from 58.21% to 95.05% and oil yield from 6.07 to 9.89 g/100 g GSP. The total phenolic content in oils obtained with cellulase pretreatment ranged from 148.7 to 279.2 mg GAE/kg and was higher compared to 127.5 mg GAE/kg determined in the control oil. Cellulase pretreatment also increased the antioxidant capacity of the oils, particularly in the oil treated at the enzyme-to-substrate ratio of 0.5 g/100 g for 18 h, which showed the highest values in the DPPH and ABTS assays (1.81 and 0.128 mmol TE/kg oil, respectively). The oils maintained low moisture content (0.47–0.48 g/100 g), moderate acidity index (2.00–2.89 mg KOH/g), acceptable peroxide values (7.24–11.24 meq O2/kg), and stable density (0.92 g/mL). The fatty acid profile remained unchanged, dominated by linoleic acid (69.8% of total fatty acids). Overall, cellulase pretreatment effectively increased oil yield and improved the chemical and functional quality of grape seed oil without compromising its physicochemical stability.
ACKNOWLEDGEMENTS
The authors express their deepest gratitude to the Universidad Nacional Agraria La Molina for providing its facilities for the development of this study. Furthermore, the valuable contribution of Eng. Cesar Moya García, during the extraction and physicochemical characterization of the analyzed samples, is recognized and highly appreciated.
FUNDING
This research was funded by the National Council for Science, Technology and Technological Innovation of Peru (CONCYTEC).
CONFLICT OF INTEREST
The authors declare no conflicts of interest.
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