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Variation in the Lipid Profile of Pacific Oyster (Crassostrea gigas) Cultured in Khanh Hoa Coast, Vietnam, Based on Location and Harvest Period
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Faculty of Food Technology, Nha Trang University, 02 Nguyen Dinh Chieu, Nha Trang, Vietnam
Submission date: 2023-10-10
Acceptance date: 2024-01-08
Online publication date: 2024-01-26
Publication date: 2024-01-26
Corresponding author
Minh Van Nguyen   

Faculty of Food Technology, Nha Trang University, Viet Nam
Pol. J. Food Nutr. Sci. 2024;74(1):16-25
The lipid profiles of the oyster Crassostrea gigas cultured in Khanh Hoa coast at three locations and harvested in different months throughout the year were compared. Seasonal and locational changes in total lipid (TL) content, phospholipid content, free fatty acid content, fatty acid (FA) composition, and thrombogenicity index were found. The total lipid content and n-3/n-6 ratio in oysters from Ninh Hoa were higher compared to those from Cam Lam and Van Ninh. For oyster from all three farming areas, the total lipid content was the lowest during the two spawning seasons (May and September). Lipids from C. gigas were separated into neutral and polar lipid fractions using silica gel column chromatography. The amount of neutral lipids recovered from the oyster muscle was significantly higher than that of the polar lipids. The FA composition showed that C. gigas was abundant in saturated fatty acids (30.89–39.16 g/100 g TL), followed by polyunsaturated fatty acids (28.13–35.88 g/100 g TL), and monounsaturated fatty acids (19.32–23.75 g/100 g TL). The dominant polyunsaturated fatty acids of oysters from the three farming areas were eicosapentaenoic acid (9.09–13.77 g/100 g TL) and docosahexaenoic acid (6.71–16.47 g/100 g TL). Based on the present findings, it can be concluded that the Pacific oyster C. gigas cultured in Khanh Hoa, Vietnam is a promising source of highly nutritious exploitable lipids.
The authors are grateful to the staff and students of the Faculty of Food Technology, Nha Trang University for their kind assistance.
This research was funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under Grant Number 106.05-2019.341.
The authors declare that they have no conflict of interest or competing interests.
Abad, M., Ruiz, C., Martinez, D., Mosquera, G., Sánchez, J. (1995). Seasonal variations of lipid classes and fatty acids in flat oyster, Ostrea edulis, from San Cibran (Galicia, Spain). Comparative Biochemistry and Physiology Part C: Pharmacology, Toxicology and Endocrinology, 110(2), 109-118.
Anjos, C., Baptista, T., Joaquim, S., Mendes, S., Matias, A.M., Moura, P., Simões, T., Matias, D. (2017). Broodstock conditioning of the Portuguese oyster (Crassostrea angulata, Lamarck, 1819): Influence of different diets. Aquaculture Research, 48(7), 3859-3878.
AOCS. (2017). In Official Methods and Recommended Practices of the American Oil Chemists Society, 5th edition. Champaign, Il, USA: AOCS Press.
Asha, K.K., Anandan, R., Mathew, S., Lakshmanan, P.T. (2014). Biochemical profile of oyster Crassostrea madrasensis and its nutritional attributes. The Egyptian Journal of Aquatic Research, 40(1), 35-41.
Bernárdez, M., Pastoriza, L., Sampedro, G., Herrera, J.J.R., Cabo, M.L. (2005). Modified method for the analysis of free fatty acids in fish. Journal of Agricultural and Food Chemistry, 53(6), 1903-1906.
Bhardwaj, K., Verma, N., Trivedi, R.K., Bhardwaj, S., Shukla, N. (2016). Significance of ratio of omega-3 and omega-6 in human health with special reference to flaxseed oil. International Journal of Biological Chemistry, 10(1-4), 1-6.
Bligh, E.G., Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8), 911-917.
Botta, R., Asche, F., Borsum, J.S., Camp, E.V. (2020). A review of global oyster aquaculture production and consumption. Marine Policy, 117, art. no. 103952.
Chakraborty, K., Chakkalakal, S.J., Joseph, D., Joy, M. (2016). Nutritional composition of edible oysters (Crassostrea madrasensis L.) from the southwest coast of India. Journal of Aquatic Food Product Technology, 25(8), 1172-1189.
Chu, F.L.E., Greaves, J. (1991). Metabolism of palmitic, linoleic, and linolenic acids in adult oysters, Crassostrea virginica. Marine Biology, 110, 229-236.
Dagorn, F., Couzinet-Mossion, A., Kendel, M., Beninger, P.G., Rabesaotra, V., Barnathan, G., Wielgosz-Collin, G. (2016). Exploitable lipids and fatty acids in the invasive oyster Crassostrea gigas on the French Atlantic coast. Marine Drugs, 14(6), art. no. 104.
De La Parra, A.M., García, O., San Juan, F.S. (2005). Seasonal variations on the biochemical composition and lipid classes of the gonadal and storage tissues of Crassostrea gigas (Thunberg, 1794) in relation to the gametogenic cycle. Journal of Shellfish Research, 24(2), 457-467.
Deranieh, R.M., Joshi, A.S., Greenberg, M.L. (2013). Thin-Layer Chromatography of Phospholipids. In: Rapaport, D., Herrmann, J. (Eds). Membrane Biogenesis. Methods in Molecular Biology, Humana Press, Totowa, NJ, 1033, pp. 21-27.
Dridi, S., Romdhane, M.S., Elcafsi, M. (2007). Seasonal variation in weight and biochemical composition of the Pacific oyster, Crassostrea gigas in relation to the gametogenic cycle and environmental conditions of the Bizert Lagoon, Tunisia. Aquaculture, 263(1-4), 238-248.
FAO. (2022). The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation of the Food and Agriculture Organization of the United Nations. Rome, FAO.
Fokina, N., Storhaug, E., Bakhmet, I., Maximovich, N., Frantzen, M., Nahrgang, J. (2018). Seasonal changes in lipid class content in mussels Mytilus spp. from Rakkfjorden in the Norwegian Sea and Kandalaksha Bay of the White Sea. Polar Biology, 41(10), 2103-2117.
Futagawa, K., Yoshie-Stark, Y., Ogushi, M. (2011). Monthly variation of biochemical composition of Pacific oysters Crassostrea gigas from two main cultivation areas in Japan. Fisheries Science, 77(4), 687-696.
Hurtado, M.A., Racotta, I.S., Arcos, F., Morales-Bojórquez, E., Moal, J., Soudant, P., Palacios, E. (2012). Seasonal variations of biochemical, pigment, fatty acid, and sterol compositions in female Crassostrea corteziensis oysters in relation to the reproductive cycle. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 163(2), 172-183.
Kandemir, Ş., Polat, N. (2007). Seasonal variation of total lipid and total fatty acid in muscle and liver of rainbow trout (Oncorhynchus mykiss W, 1792) reared in Derbent Dam Lake. Turkish Journal of Fisheries and Aquatic Sciences, 7(1), 27-31.
Lan, N.T., Kovyazin, V.F., Huan, P.T. (2021). Phytoplankton composition of the mangrove ecosystem in Khanh Hoa province, Vietnam. IOP Conference Series: Earth and Environmental Science, 876(1), art. no. 012061.
Linehan, L.G., O'Connor, T.P., Burnell, G. (1999). Seasonal variation in the chemical composition and fatty acid profile of Pacific oysters (Crassostrea gigas). Food Chemistry, 64(2), 211-214.
Lira, G.M., Pascoal, J.C.M., Torres, E.A.F.S., Soares, R.A.M., Mendonça, S., Sampaio, G.R., Correia, M.S., Cabral, C.C.V.Q., Cabral Júnior, C.R., López, A.M.Q. (2013). Influence of seasonality on the chemical composition of oysters (Crassostrea rhizophorae). Food Chemistry, 138(2-3), 786-790.
Liu, Y.F., Wu, Z.X., Zhang, J., Liu, Y.X., Liu, Z.Y., Xie, H.K., Rakariyatham, K., Zhou, D.Y. (2020). Seasonal variation of lipid profile of oyster Crassostrea talienwhanensis from the Yellow Sea area. Journal of Aquatic Food Product Technology, 29(4), 360-372.
Martino, R.C., Cruz, G.M.D. (2004). Proximate composition and fatty acid content of the mangrove oyster Crassostrea rhizophorae along the year seasons. Brazilian Archives of Biology and Technology, 47(6), 955-960.
Napolitano, G.E., Ackman, R.G. (1992). Anatomical distributions and temporal variations of lipid classes in sea scallops Placopecten magellanicus (gmelin) from Georges Bank (Nova Scotia). Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 103(3), 645-650.
Pazos, A.J., Ruíz, C., García-Martin, O., Abad, M., Sánchez, J. (1996). Seasonal variations of the lipid content and fatty acid composition of Crassostrea gigas cultured in E1 Grove, Galicia, NW Spain. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 114(2), 171-179.
Pernet, F., Pelletier, C.J., Milley, J. (2006). Comparison of three solid-phase extraction methods for fatty acid analysis of lipid fractions in tissues of marine bivalves. Journal of Chromatography A, 1137(2), 127-137.
Pogoda, B., Buck, B.H., Saborowski, R., Hagen, W. (2013). Biochemical and elemental composition of the offshore-cultivated oysters Ostrea edulis and Crassostrea gigas. Aquaculture, 400-401, 53-60.
Qin, Y., Li, X., Li, J., Zhou, Y., Xiang, Z., Ma, H., Noor, Z., Mo, R., Zhang, Y., Yu, Z. (2021). Seasonal variations in biochemical composition and nutritional quality of Crassostrea hongkongensis, in relation to the gametogenic cycle. Food Chemistry, 356, art. no. 129736.
Rato, A., Pereira, L.F., Joaquim, S., Gomes, R., Afonso, C., Cardoso, C., Machado, J., Gonçalves, J.F.M., Vaz-Pires, P., Magnoni, L.J., Matias, A.M., Matias, D., Bandarra, N.M., Ozório, R.O.A. (2019). Fatty acid profile of pacific oyster, Crassostrea gigas, fed different ratios of dietary seaweed and microalgae during broodstock conditioning. Lipids, 54(9), 531-542.
Saito, H., Marty, Y. (2010). High levels of icosapentaenoic acid in the lipids of oyster Crassostrea gigas ranging over both Japan and France. Journal of Oleo Science, 59(6), 281-292.
Soudant, P., Van Ryckeghem, K., Marty, Y., Moal, J., Samain, J.F., Sorgeloos, P. (1999). Comparison of the lipid class and fatty acid composition between a reproductive cycle in nature and a standard hatchery conditioning of the Pacific Oyster Crassostrea gigas. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 123(2), 209-222.
Stewart, J.C.M. (1980). Colorimetric determination of phospholipids with ammonium ferrothiocyanate. Analytical Biochemistry, 104(1), 10-14.
Su, X.Q., Antonas, K., Li, D., Nichols, P. (2006). Seasonal variations of total lipid and fatty acid contents in the muscle of two Australian farmed abalone species. Journal of Food Lipids, 13(4), 411-423.
Swanson, D., Block, R., Mousa, S.A. (2012). Omega-3 fatty acids EPA and DHA: health benefits throughout life. Advances in Nutrition, 3(1), 1-7.
Truong, T., Nguyen, C., Lam, N.N., Jensen, K. (2014). Seasonal and spatial distribution of mesozooplankton in a tropical estuary, Nha Phu, South Central Viet Nam. Biologia, 69(1), 80-91.
Tung, N.T., Son, T.P.H. (2019). GIS-based multi-criteria evaluation models for selection of suitable sites for Pacific oyster (Crassostrea gigas) aquaculture in the central region of Vietnam. Journal of Environmental Science and Engineering A, 8, 141-158.
Ugalde, S.C., Vu, S.V., Giang, C.T., Ngoc, N.T.H., Tran, T.K.A., Mullen, J.D., Vu, V.I, O'Connor, W. (2023). Status, supply chain, challenges, and opportunities to advance oyster aquaculture in northern Vietnam. Aquaculture, 572, art. no. 739548.
Ulbricht, T.L.V., Southgate, D.A.T. (1991). Coronary heart disease: seven dietary factors. The Lancet, 338(8773), 985-992.
Viladrich, N., Bramanti, L., Tsounis, G., Chocarro, B., Martínez-Quitana, A., Ambroso, S., Madurell, T., Rossi, S. (2016). Variation in lipid and free fatty acid content during spawning in two temperate octocorals with different reproductive strategies: surface versus internal brooder. Coral Reefs, 35, 1033-1045.
Fatty Acid Composition and Anticancer Activity of Neutral and Polar Lipids of Pacific Oyster (Crassostrea gigas) Cultured in Khanh Hoa Coast in Vietnam
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Polish Journal of Food and Nutrition Sciences
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