ANTIOXIDANT ACTIVITY OF PHENOLIC FRACTIONS OF  LITSEA MONOPETALA  (PERSIMON-LEAVED LITSEA) BARK EXTRACT.

Phenolic compounds were extracted from Litsea monopetala bark using methanol. Four fractions (I-IV) were separated from extract on a Sephadex LH-20 column using methanol as the mobile phase evaluated for their total antioxidant activity, antiradical activity against DPPH·, (2,2-diphenyl-1-picrylhydrazyl radical), and reducing power. The total phenolics and tannin contents of the fractions were determined. The highest relative content in the crude extract was found for fraction I. The content of total phenolics in fractions ranged from 169 mg/g (fraction I) to 753 mg/g (fraction II). Condensed tannins were detected in fractions II-IV. The fractions exhibited the total antioxidant activity from 1.90 mmol Trolox/g (fraction I) to 7.06 mmol Trolox/g (fraction IV). Fraction III was characterised by the highest antiradical activity against DPPH (EC₅₀ = 0.011 mg/assay). Fractions II and III showed the strongest reducing power which was app. four times higher than that of fractions I. The presence of three main phenolic compounds in fraction I was confirmed using RP-HPLC.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.