ORIGINAL ARTICLE
Phenolic Composition, and Antioxidant and Antineurodegenerative Potential of Methanolic Extracts of Fruit Peel and Flesh of Pear Varieties from Serbia
 
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1
Department for Education and Communication, Natural History Museum in Belgrade, Njegoševa 51, 11000 Belgrade, Serbia
2
Institute of Botany and Botanical Garden "Jevremovac", Takovska 43, 11000 Belgrade, Serbia, University of Belgrade - Faculty of Biology, Serbia
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Institute for Medicinal Plant Research “Dr. Josif Pančić”, Institute for Medicinal Plant Research “Dr. Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade, Serbia
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Department of Ecology, University of Belgrade, Institute for Biological Research ‘Siniša Stanković’, Bulevar Despota Stefana 142, Belgrade, Serbia
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Department of experimental and applied botany, University of Belgrade - Faculty of Biology, Institute of Botany and Botanical Garden "Jevremovac", Takovska 43, 11000 Belgrade, Serbia
CORRESPONDING AUTHOR
Aleksandra Savić   

Department for Education and Communication, Natural History Museum in Belgrade, Njegoševa 51, 11000 Belgrade, Serbia
Submission date: 2021-01-22
Final revision date: 2021-05-29
Acceptance date: 2021-05-31
Online publication date: 2021-06-10
Publication date: 2021-06-10
 
Pol. J. Food Nutr. Sci. 2021;71(2):225–236
 
KEYWORDS
TOPICS
ABSTRACT
Pear (Pyrus communis L.) is consumed as fresh fruit, in numerous food products, and also used as a traditional remedy in various countries, including Serbia. In search of bioactive compounds, six traditional pear varieties (‘Vidovača’, ‘Lubeničarka’, ‘Karamanka’, ‘Jeribasma’, ‘Lončara’, ‘Takiša’) and wild pear from Serbia were investigated and compared with a commercial variety (‘Williams Bartlett’). The aim of this study was to determine the total phenolic and flavonoid contents, phenolic composition, antioxidant capacity, and antineurodegenerative activities of methanolic extracts of peel, flesh, and mixed peel and flesh of pear fruits. Phenolic composition of extracts was determined with HPLC-DAD, while the antioxidant activity of extracts was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric-reducing antioxidant power (FRAP) assays. Bearing in mind that oxidative stress is closely linked to neurodegeneration, the antineurodegenerative potential of the extracts was assessed by the inhibition of acetycholineserase (AChE) and tyrosinase (TYR) activities. The extracts of traditional varieties, particularly peel extracts, had a high content of phenolics, as well as significant antioxidant and moderate antineurodegenerative potential, compared to the commercial variety. The highest contents of total flavonoids and individual compounds, such as arbutin and chlorogenic acid, as well as the strongest antioxidant and TYR inhibitory activities were reported for the ‘Takiša’ peel extract. The performed analyses have revealed that fruits of traditional Serbian pear varieties are rich in bioactive components and could be used as functional food and for possible nutraceutical applications, to prevent diseases induced by oxidative stress.
ABBREVIATIONS
TPC, total phenolic content; TFC, total flavonoid content; DPPH, 2,2-diphenyl-1-picrylhydrazyl; ABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); FRAP, ferric-reducing antioxidant power; BHA, 2(3)-tert-butyl-4-hydroxyanisole; BHT, 3,5-di-tert-butyl-4-hydroxytoluene; TPTZ, 2,4,6-tripyridyl-S-triazine; L-DOPA, 3,4-dihydroxy-L-phenylalanine; AAE, ascorbic acid equivalents; DTNB, 5,5′-dithiobis(2-nitrobenzoic acid); AChE, acetylcholinesterase; TYR, tyrosinase; HPLC-DAD, high-performance liquid chromatography with diode array detection.
ACKNOWLEDGEMENTS
The authors are grateful to Bora Milićević for drawings of Pyrus varieties.
FUNDING
This manuscript was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Grants No 451-03-9/2021-14/200178, 451-03-9/2021-14/200003 and 451-03-9/2021-14/200007.
 
REFERENCES (41)
1.
Abaci, Z.T., Sevindik, E., Ayvaz, M. (2016). Comparative study of bioactive components in pear genotypes from Ardahan/Turkey. Biotechnology & Biotechnological Equipment, 30, 36-43. https://doi.org/10.1080/131028....
 
2.
Adem, S., Eyupoglu, V., Sarfraz, I., Rasul, A., Ali, M. (2020). Identification of potent COVID-19 main protease (Mpro) inhibitors from natural polyphenols: An in silico strategy unveils a hope against CORONA. Preprints, art. no. 2020030333. https://doi.org/10.20944/prepr....
 
3.
Benzie, I.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Analytical Biochemistry, 239, 70–76. https://doi.org/10.1006/abio.1....
 
4.
Blois, M.S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181, 1199–1200. https://doi.org/10.1038/181119....
 
5.
Brahem, M., Renard, C.M.G.C., Eder, S., Loonis, M., Ouni, R., Messaoud, M., Le Bourvellec, C. (2017). Characterization and quantification of fruit phenolic compounds of European and Tunisian pear cultivars. Food Research International, 95, 125-133. https://doi.org/10.1016/j.food....
 
6.
Carbonaro, M., Mattera, M., Nicoli, S., Bergamo, P., Cappelloni, M. (2002). Modulation of antioxidant compounds in organic vs conventional fruit (Peach, Prunus persica L., and Pear, Pyrus communis L.). Journal of Agricultural and Food Chemistry, 50, 5458-5462. https://doi.org/10.1021/jf0202....
 
7.
Chel-Guerrero L.D., Sauri-Duch E., Fragoso-Serrano M.C., Pérez-Flores L.J., Gómez-Olivares J.L., Salinas-Arreortu, N., Sierra-Palacios, E.C., Mendoza-Espinoza, J.A. (2018). Phytochemical profile, toxicity, and pharmacological potential of peels from four species of tropical fruits. Journal of Medicinal Food, 21(7), 734-743. https://doi.org/10.1089/jmf.20....
 
8.
Cory, H., Passarelli, S., Szeto, J., Tamez, M., Mattei, J. (2018). The role of polyphenols in human health and food systems: A mini-review. Frontiers in Nutrition, 5, 87-96. https://doi.org/10.3389/fnut.2....
 
9.
Dadgar M., Pouramir, M., Dastan, Z., Ghasemi-Kasman, M., Ashrafpour, M., Moghadamnia, A.A., Khafri, S., Pourghasem, M. (2018). Arbutin attenuates behavioral impairment and oxidative stress in an animal model of Parkinson's disease. Avicenna Journal of Phytomedicine, 8, 533-542.
 
10.
Dajić-Stevanović, Z., Petrović, M., Aćić, S. (2014). Ethnobotanical knowledge and traditional use of plants in Serbia in relation to sustainable rural development. In: Pieroni, A., Quave, C.L. (Eds.), Ethnobotany and Biocultural Diversities in the Balkans, Springer, New York, Heidelberg, Dordrecht, London, pp. 229-252. https://doi.org/10.1007/978-1-....
 
11.
Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7, 88–95. https://doi.org/10.1016/0006-2....
 
12.
Galanakis, C.M., Rizou, M., Aldawoud, T.M., Ucak, I., Rowan, N.J. (2021). Innovations and technology disruptions in the food sector within the COVID-19 pandemic and post-lockdown era. Trends in Food Science & Technology, 110, 193-200. https://doi.org/10.1016/j.tifs....
 
13.
Hammer, O., Harper, D.T., Ryan, P.D. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica, 4, 1-9.
 
14.
Imeh, U., Khokhar, S. (2002). Distribution of conjugated and free phenols in fruits. Journal of Agricultural and Food Chemistry, 50, 6301-6306. https://doi.org/10.1021/jf0203....
 
15.
Jeong, D.E., Cho, J.Y., Lee, Y.G., Jeong, H.Y., Lee, H.J., Moon, J.H. (2017). Isolation of five proanthocyanidins from pear (Pyrus pyrifolia Nakai) fruit peels. Food Science and Biotechnology, 26(5), 1209-1215. https://doi.org/10.1007/s10068....
 
16.
Kaur, R., Arya, V. (2012). Ethnomedicinal and phytochemical perspectives of Pyrus communis L. Journal of Pharmacognosy and Phytochemistry, 1, 14-19.
 
17.
Kolniak-Ostek, J. (2016). Chemical composition and antioxidant capacity of different anatomical parts of pear (Pyrus communis L.). Food Chemistry, 203, 491–497. https://doi.org/10.1016/j.food....
 
18.
Kolniak-Ostek, J., Kłopotowska, D., Rutkowski, K.P., Skorupińska, A., Kruczyńska, D.E. (2020). Bioactive compounds and health-promoting properties of pear (Pyrus communis L.) fruits. Molecules, 25(19), art. no. 4444. https://doi.org/10.3390/molecu....
 
19.
Li, X., Jiang, Q., Wang, T., Liu, J., Chen, D. (2016a). Comparison of the antioxidant effects of quercitrin and isoquercitrin: Understanding the role of the 6 ″-OH group. Molecules, 21(9), 1246-1257. https://doi.org/10.3390/molecu....
 
20.
Li, X., Wang, T., Gao, W. (2016b). Nutritional composition of pear cultivars (Pyrus spp.). In V.R. Preedy, M.S.J. Simmonds (Eds.) Nutritional Composition of Fruit Cultivars, Academic Press, San Diego, CA, USA, pp. 573–608. https://doi.org/10.1016/B978-0....
 
21.
Li, X., Wang, T., Zhou, B., Gao, W., Cao, J., Huang, L. (2014). Chemical composition and antioxidant and anti-inflamatory potential of peels and flesh from 10 different pear varieties (Pyrus sp.). Food Chemistry, 152, 531-538. https://doi.org/10.1016/j.food....
 
22.
Li, X., Zhang, J.Y., Gao, V.V.Y., Wang, H.Y., Cao, J.G., Huang, L.Q. (2012). Chemical composition and anti-inflammatory and antioxidant activities of either pear cultivars. Journal of Agricultural and Food Chemistry, 60, 8738-8744. https://doi.org/10.1021/jf3032....
 
23.
Liaudanskas, M., Zymonė, K., Viškelis, J., Klevinskas, A., Janulis, V. (2017). Determination of the phenolic composition and antioxidant activity of pear extracts. Journal of Chemistry, 2017(1), 1-9. https://doi.org/10.1155/2017/7....
 
24.
Lin, L.Z., Harnly, J.M. (2008). Phenolic compounds and chromatographic profiles of pear skins (Pyrus spp.). Journal of Agricultural and Food Chemistry, 56, 9094–9101. https://doi.org/10.1021/jf8013....
 
25.
Ma, J.-N.., Wang, S.-L., Zhang, K., Wu, Z.-G., Hattori, M., Chen, G.-L., Ma, C.-M. (2012). Chemical components and antioxidant activity of the peels of commercial apple shaped pear (fruit of Pyrus pyrifolia cv. pingguoli). Journal of Food Science, 77, C1097-C1102. https://doi.org/10.1111/j.1750....
 
26.
Masuda, T., Yamashita, D., Takeda, Y., Yonemori, S. (2005). Screening for tyrosinase inhibitors among extracts of seashore plants and identification of potent inhibitors from Garcinia subelliptica. Bioscience, Biotechnology and Biochemistry, 69, 197–201. https://doi.org/10.1271/bbb.69....
 
27.
McClure, K.A., Gong, Y., Song, J., Vinqvist-Tymchuk, M., Palmer, L.C., Fan, L., Burgher-MacLellan, K., Zhang, Z.Q., Celton, J.M., Forney, C.F., Migicovsky, Z., Myles, S. (2019). Genome-wide association studies in apple reveal loci of large effect controlling apple polyphenols. Horticulture Research, 6(1), art. no. 107. https://doi.org/10.1038/s41438....
 
28.
Miller, N.J., Rice-Evans, C., Davies, M.J., Gopinathan, V., Milner, A. (1993). A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Science, 84, 407–412. https://doi.org/10.1042/cs0840....
 
29.
Öztürk, A., Demirsoy, L., Demirsoy, H., Asan, A., Gül, O. (2015). Phenolic compounds and chemical characteristics of pears (Pyrus communis L.). International Journal of Food Properties, 18, 536–546. https://doi.org/10.1080/109429....
 
30.
Park, Y.K., Koo, M.H., Ikegaki, M., Contado, J.L. (1997). Comparison of the flavonoid aglycone contents of Apis melifera propolis from various regions of Brazil. Arquivos de Biologia e Tecnologia, 40, 97-106.
 
31.
Parle, M., Arzoo, (2016). Why is pear so dear. International Journal of Research in Ayurveda and Pharmacy, 7, 108-113. https://doi.org/10.7897/2277-4....
 
32.
Reiland, H., Slavin, J. (2015). Systematic review of pears and health. Food and Nutrition Today, 50(6), 301-305. https://doi.org/10.1097/NT.000....
 
33.
Salta, J, Martins, A., Santos, R.G., Neng, N.R., Nogneira, J.M.F., Justino, J., Ruter, A.P. (2010). Phenolic composition and antioxidant activity of Rocha pear and other pear cultivars – a comparative study. Journal of Functional Foods, 2, 153-157. https://doi.org/10.1016/j.jff.....
 
34.
Savić, A., Jarić, S., Dajić-Stevanović, Z., Duletić-Laušević, S. (2019). Ethnobotanical study and traditional use of autochthonous pear varieties (Pyrus communis L.) in southwest Serbia (Polimlje). Genetic Resources and Crop Evolution, 66, 589-609. https://doi.org/10.1007/s10722....
 
35.
Sharma, K., Pasricha, V., Satpathy, G., Gupta, R.K. (2015). Evaluation of phytochemical activity of raw Pyrus communis L, an underexploited fruit. Journal of Pharmacognosy and Phytochemistry, 3, 46-50.
 
36.
Singleton, V.L., Rossi, J.A. (1965). Colometric of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16, 144–158.
 
37.
Szwajgier, D., Borowiec, K. (2012). Screening for cholinesterase inhibitors in selected fruits and vegetables. Electronic Journal of Polish Agricultural Universities, 15, art. no. 6.
 
38.
Šavikin, K., Živković, J., Zdunjić, G., Dojčinović, B., Menković, N. (2014). Phenolic and mineral profiles of four Balkan indigenous apple cultivars monitored at two different maturity stages. Journal of Food Composition and Analysis, 35, 101-111. https://doi.org/10.1016/j.jfca....
 
39.
Šavikin, K., Živković J., Alimpić, A., Zdunjić, G., Janković T., Duletić-Laušević, S., Menković, N. (2018). Activity guided fractionation of pomegranate extract and its antioxidant, antidiabetic and antineurodegenerative properties. Industrial Crops & Products, 113, 142–149. https://doi.org/10.1016/j.indc....
 
40.
Taylor, R. (1990). Interpretation of the correlation coefficient: a basic review. Journal of Diagnostic Medical Sonography, 6(1), 35-39. https://doi.org/10.1177/875647....
 
41.
Wilson, D.W., Nash, P., Buttar, H.S., Griffiths, K., Singh, R., De Meester, F., Takahashi, T. (2017). The role of food antioxidants, benefits of functional foods, and influence of feeding habits on the health of the older person: an overview. Antioxidants, 6(4), art. no. 81. https://doi.org/10.3390/antiox....
 
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